Lysinibacillus antri sp. nov., isolated from cave soil.
Lysinibacillus antri sp. nov., isolated from cave soil.
复制标题
DOI：
10.1099/ijsem.0.004169
复制
发表时间：
2020-04
影响因子：
2.8
通讯作者：
Manik Prabhu Narsing Rao;Zhou-Yan Dong;Xue-Ke Niu;Kun Zhang;YING-QIAN Kang;Awalagaway Dhulappa;Min Xiao;Wenjun Li
中科院分区：
文献类型：
--
作者：
Manik Prabhu Narsing Rao;Zhou-Yan Dong;Xue-Ke Niu;Kun Zhang;YING-QIAN Kang;Awalagaway Dhulappa;Min Xiao;Wenjun Li
从贵州省兴义县喀斯特溶洞土壤样品中分离到一株革兰氏染色阳性、可运动、棒状、内孢子形成的菌株SYSU K30002T。SYSU K30002T在28-40°C（37°C）、pH 5.0-8.0 （pH 7.0）和0- 4% (w/v) NaCl（无NaCl时最佳）条件下生长。细胞壁肽聚糖类型为A4α (Lys-Asp)。SYSU K30002T细胞壁糖为核糖、半乳糖和甘露糖，MK-7为甲萘醌。主要脂肪酸为iso-C15: 0、C16: 1 ω7c醇和iso-C16: 0。极性脂质为二磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺和两种未识别的磷脂。基因组DNA中G+C含量为36.1 mol%。SYSU K30002T与近缘种的平均核苷酸同源性低于物种划分的临界值（95 ~ 96%）。基于表型、化学分类和基因组比较，菌株SYSU K30002T为Lysinibacillusantri sp. nov.的新种，建议命名为Lysinibacillusantri sp. 11。型应变为SYSU K30002T （=KCTC 33955T=CGMCC 1.13504T）。
A Gram-stain-positive, motile, rod-shaped and endospore-forming strain, SYSU K30002T, was isolated from a soil sample collected from a karst cave in Xingyi county, Guizhou province, south-west China. SYSU K30002T grew at 28-40 °C (optimum, 37 °C), at pH 5.0-8.0 (optimum, pH 7.0) and in the presence of 0-4 % (w/v) NaCl (optimum in the absence of NaCl). The cell-wall peptidoglycan type was A4α (Lys-Asp). The cell-wall sugars of SYSU K30002T were ribose, galactose and mannose, and MK-7 was the menaquinone. The major fatty acids were iso-C15 : 0, C16 : 1 ω7c alcohol and iso-C16 : 0. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and two unidentified phospholipids. The G+C content of the genomic DNA was 36.1 mol%. The average nucleotide identity values between SYSU K30002T and its closest relatives were below the cut-off level (95-96 %) for species delineation. Based on phenotypic, chemotaxonomic and genome comparisons, strain SYSU K30002T represents a novel species of the genus Lysinibacillus, for which the name Lysinibacillusantri sp. nov. is proposed. The type strain is SYSU K30002T (=KCTC 33955T=CGMCC 1.13504T).