Localization of Escherichia coli poly(A) polymerase I in cellular membrane
Localization of Escherichia coli poly(A) polymerase I in cellular membrane
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DOI：
10.1016/j.bbrc.2005.02.025
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发表时间：
2005-04-08
影响因子：
3.1
通讯作者：
Wegrzyn, G
中科院分区：
文献类型：
--
作者：
Jasiecki, J;Wegrzyn, G
聚(A)聚合酶I （PAP I）是pcnB基因产物，是大肠杆菌中负责RNA聚腺苷化的主要酶。聚腺苷化的RNA分子被称为RNA降解体的多蛋白复合物迅速降解。本研究表明，除了存在于细胞质中，PAP 1也存在于细胞膜中。虽然这一观察结果可能令人惊讶，但最近有人证明大肠杆菌RNA降解体也与细胞质膜有关。此外，我们发现单链RNA噬菌体MS2和Q β的发育，而不是单链DNA噬菌体M13，在pcnB突变体中比在其他等基因pcnB（+）宿主中更有效。(c) 2005爱思唯尔公司版权所有。
Poly(A) polymerase I (PAP I), the pcnB gene product, is the main enzyme responsible for RNA polyadenylation in Escherichia coli. Polyadenylated RNA molecules are rapidly degraded by a multiprotein complex called RNA degradosome. Here we demonstrate that apart from its presence in cytosol, PAP I is also localized in cellular membrane. Although this observation might appear surprising, it was demonstrated recently by others that E. coli RNA degradosome is also associated with the cytoplasmic membrane. Moreover, we show that development of single-stranded RNA bacteriophages MS2 and Q beta, but not that of single-stranded DNA bacteriophage M13, is more efficient in the pcnB mutant relative to an otherwise isogenic pcnB(+) host. (c) 2005 Elsevier Inc. All rights reserved.