Mineral Trioxide Aggregate Induces Bone Morphogenetic Protein-2 Expression and Calcification in Human Periodontal Ligament Cells
Mineral Trioxide Aggregate Induces Bone Morphogenetic Protein-2 Expression and Calcification in Human Periodontal Ligament Cells
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DOI：
10.1016/j.joen.2009.12.024
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发表时间：
2010-04-01
影响因子：
4.2
通讯作者：
Akamine, Akifumi
中科院分区：
文献类型：
--
作者：
Maeda, Hidefumi;Nakano, Tsuguhisa;Akamine, Akifumi
前言：三氧化矿物聚集体(MTA)是一种治疗性的牙髓修复材料，据报道具有钙化组织传导活性，但其机制尚不清楚。我们推测，MTA中钙离子的溶解可能在人牙周韧带细胞(HPLC)的成骨/成牙骨质分化过程中起重要作用。方法：取2例HPLC，分别在有或无MTA盘和/或CaCl2的条件下培养，观察钙释放、钙化活性、钙敏感受体(CaSR)基因表达和骨形态发生蛋白-2(BMP-2)的表达，以及BMP-2受体蛋白和基因的表达。结果：MTA在14d内可大量释放钙(4 mm o l/L)。4周后，两组HPLC分别在MTA附近出现钙化和BMP-2分布。HPLC固有地表达编码CaSR和BMP-2受体的基因。外源性CaCl2可诱导HPLC CaSR基因表达，并在整个培养过程中促进钙化和BMP-2的合成，而MgCl2对此无影响。MTA和CaCl2均刺激BMP-2基因表达高于基础水平。结论：我们首次报道了HPLC与MTA直接共培养可上调BMP2的表达和钙化。这些结果可能是通过CaSR相互作用，潜在地被激活的钙从MTA释放到培养环境中。(J Endod 2010；36：647-652)
Introduction: Mineral trioxide aggregate (MTA) is a therapeutic, endodontic repair material that is reported to exhibit calcified tissue-conductive activity although the mechanisms remain unclear. We hypothesize that the dissolution of calcium from MTA into the surrounding environment may play an important role in the osteoblastic/cementoblastic differentiation of human periodontal ligament cells (HPLCs). Methods: Two populations of HPLCs were obtained from two patients, respectively, and were cultured in the presence or absence of MTA discs and/or CaCl2 in order to investigate calcium release, calcification activity, calcium-sensing receptor (CaSR) gene expression and bone morphogenetic protein-2 (BMP-2), and BMP-2 receptor protein and gene expression. Results: MTA released a substantial accumulation of calcium (4 mmol/L) within 14 days into culture media. After 4 weeks, the two populations of HPLCs independently exhibited calcification as well as BMP-2 distribution in the vicinity of MTA. HPLCs inherently expressed genes encoding for the CaSR and BMP-2 receptors. Exogenous CaCl2 media supplementation induced CaSR gene expression in HPLCs and calcification and BMP-2 synthesis throughout the entire HPLC cultures, whereas MgCl2 had no effect. Both MTA and CaCl2 stimulated BMP-2 gene expression above that of baseline levels. Conclusion: Here we show the first report showing that HPLCs cocultured directly with MTA up-regulated BMP2 expression and calcification. These results may be through CaSR interactions that were potentially activated by the release of calcium from MTA into the culture environment. (J Endod 2010;36:647-652)