Telomere length determined by the fluorescence in?situ hybridisation distinguishes malignant and benign cells in cytological specimens
Telomere length determined by the fluorescence in?situ hybridisation distinguishes malignant and benign cells in cytological specimens
复制标题
荧光原位杂交测定的端粒长度可区分细胞学标本中的恶性和良性细胞
作者：
Matsuda Y.;Suzuki A.;Esaka S.;Hamashima Y.;Imaizumi M.;Kinoshita M.;Shirahata H.;Kiso Y.;Kojima H.;Matsukawa M.;Fujii Y.;Ishikawa N.;Aida J.;Takubo K.;Ishiwata T.;Nishimura M.;Arai T.
研究背景端粒是真核细胞染色体末端TTAGGG的串联重复序列，在防止染色体不稳定中起着关键作用。本研究的目的是用荧光原位杂交技术(FISH)测定细胞学标本的端粒长度。方法将41例脱落细胞标本涂片用于FISH。结果阳性组(恶性组，n=25)的端粒信号强度明显低于阴性组(非恶性组，n=16)，着丝粒信号强度也明显低于阴性组(n=16)。DAPI强度的差异无统计学意义。端粒/着丝粒强度比值在阳性组和阴性组之间无显著差异。腹水或胸腔积液抽吸标本(n=23)与超声引导下胰腺FNA标本(n=18)的信号强度差异无统计学意义。结论端粒长度可作为细胞学标本中良恶性细胞的鉴别指标。这一新方法可能有助于提高癌症患者的诊断水平。
BackgroundTelomeres are tandem repeats of TTAGGG at the end of eukaryotic chromosomes that play a key role in preventing chromosomal instability. The aim of the present study is to determine telomere length using fluorescence in situ hybridisation (FISH) on cytological specimens.MethodsAspiration samples (n = 41) were smeared on glass slides and used for FISH.ResultsTelomere signal intensity was significantly lower in positive cases (cases with malignancy, n = 25) as compared to negative cases (cases without malignancy, n = 16), and the same was observed for centromere intensity. The difference in DAPI intensity was not statistically significant. The ratio of telomere to centromere intensity did not show a significant difference between positive and negative cases. There was no statistical difference in the signal intensities of aspiration samples from ascites or pleural effusion (n = 23) and endoscopic ultrasound‐guided FNA samples from the pancreas (n = 18).ConclusionsThe present study revealed that telomere length can be used as an indicator to distinguish malignant and benign cells in cytological specimens. This novel approach may help improve diagnosis for cancer patients.