Embryonic origin and lineage of juxtaglomerular cells
Embryonic origin and lineage of juxtaglomerular cells
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DOI：
10.1152/ajprenal.2001.281.2.f345
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发表时间：
2001-08-01
影响因子：
4.2
通讯作者：
Gomez, RA
中科院分区：
文献类型：
--
作者：
Lopez, MLSS;Pentz, ES;Gomez, RA
为了确定肾小球旁(JG)细胞的胚胎起源和谱系，在基因标记的和野生型小鼠之间进行了胚胎肾脏移植；对不同发育阶段的肾素、平滑肌和内皮细胞进行了标记研究；以及在血管前肾脏中进行了肾素和其他细胞识别标记的单细胞RT-PCR。从胚胎肾脏第12天到第15天(E12到E15)，肾素细胞还没有表达血管和内皮标志物。在E16，肾素细胞获得了平滑肌而不是内皮标记物，这表明这些细胞与内皮谱系无关，而且平滑肌表型是JG细胞分化的后期事件。将血管前基因标记的E12小鼠肾脏移植到成年小鼠的眼前房或肾被膜下，发现后肾胚泡内的肾素细胞前体细胞原位分化为JG细胞。我们认为JG细胞来源于后肾间充质，而不是来自肾外来源。我们认为肾素细胞的分化程度低于(并有能力形成)肾小动脉的平滑肌细胞。
To define the embryonic origin and lineage of the juxtaglomerular (JG) cell, transplantation of embryonic kidneys between genetically marked and wild-type mice; labeling studies for renin, smooth muscle, and endothelial cells at different developmental stages; and single cell RT-PCR for renin and other cell identity markers in prevascular kidneys were performed. From embryonic kidney day 12 to day 15 (E12 to E15), renin cells did not yet express smooth muscle or endothelial markers. At E16 renin cells acquired smooth muscle but not endothelial markers, indicating that these cells are not related to the endothelial lineage, and that the smooth muscle phenotype is a later event in the differentiation of the JG cell. Prevascular genetically labeled E12 mouse kidneys transplanted into the anterior chamber of the eye or under the kidney capsule of adult mice demonstrated that renin cell progenitors originating within the metanephric blastema differentiated in situ to JG cells. We conclude that JG cells originate from the metanephric mesenchyme rather than from an extrarenal source. We propose that renin cells are less differentiated than (and have the capability to give rise to) smooth muscle cells of the renal arterioles.