Carcinoembryonic antigen and blood group substances in carcinomas of the stomach and colon.
Carcinoembryonic antigen and blood group substances in carcinomas of the stomach and colon.
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胃癌和结肠癌中的癌胚抗原和血型物质。
DOI：
10.1093/jnci/53.4.933
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发表时间：
1974
期刊：
影响因子：
--
通讯作者：
J. Holzner
中科院分区：
文献类型：
--
作者：
H. Denk;G. Tappeiner;A. Davidovits;R. Eckerstorfer;J. Holzner
本文用间接免疫荧光法和混合细胞凝集反应法对胃癌和结肠癌的消化系统癌胚抗原（CEA）和血型物质（BG）进行了研究。与CEA相比，BG与肿瘤的分化程度无正相关，在结肠和胃腺癌中，CEA和BG如果同时存在，则显示不同的定位。CEA在肿瘤细胞的腔定向表面上检测到，而BG分布在整个细胞上。未分化癌要么CEA阴性，要么仅含有微量CEA，但表达BG的百分比与分化良好的癌大致相同。印戒细胞定位于细胞质中的CEA始终阳性，大多数BG阴性。胃癌和结肠癌的BG活性对乙醇敏感。只有在一些主要是分化良好的腺癌没有BG抵抗在一定程度上的乙醇治疗。在我们的系统中，抗BG血清不与CEA反应，这表明形态学方法对检测交叉反应性太不敏感，或者在分离和纯化过程中未发现CEA上负责与BG-A抗血清在体外观察到的交叉反应的位点。在某些胃癌和结肠癌的冰冻切片中，检测到的BG活性与患者的BG不相容。
Carcinoembryonic antigen (CEA) of the digestive system and blood group substances (BG) were studied in gastric and colon carcinomas by indirect immunofluorescence and mixed-cell agglutination reaction, respectively. In contrast to CEA, no positive correlation between BG and the differentiation of the tumor was found. In adenocarcinomas of the colon and stomach, CEA and BG, if present simultaneously, showed different localizations. CEA was detected on the lumen-oriented surface of the tumor cells, whereas BG were distributed over the whole cell. Anaplastic carcinomas were either CEA negative or contained only minute amounts of CEA, but expressed BG in roughly the same percentage as well-differentiated carcinomas. Signet-ring cells consistently positive for CEA localized in the cytoplasm were mostly BG negative. The BG activity in gastric and colon cancers was ethanol sensitive. Only in some predominantly well-differentiated adenocarcinomas did BG resist to some degree the ethanol treatment. AntiBG serum did not react with CEA in our system, which indicates either that the morphologic methods were too insensitive to detect cross-reactivity or that sites on CEA responsible for the cross-reactions with BG-A antiserum observed in vitro were uncovered during isolation and purification. In some frozen sections of gastric and colon carcinomas, the BG activity detected was noncompatible with the BG of the patient.