Zfp312 is required for suhcortical axonal projections and dendritic morphology of deep-layer pyramidal neurons of the cerebral cortex
Zfp312 is required for suhcortical axonal projections and dendritic morphology of deep-layer pyramidal neurons of the cerebral cortex
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DOI：
10.1073/pnas.0509032102
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发表时间：
2005-12-06
影响因子：
11.1
通讯作者：
Sestan, N
中科院分区：
文献类型：
--
作者：
Chen, JG;Rasin, MR;Sestan, N
大脑皮质的锥体神经元在其轴突投射和树突形态方面表现出明显的层特异性和亚型特异性差异。在此我们表明，转录因子Zfp312由第V层和第VI层皮质下投射锥体神经元及其祖细胞选择性表达。用小干扰RNA敲低Zfp312会显著减少深层锥体神经元的皮质下轴突投射数量，并改变其树突形态。相反，在第II层和第III层皮质投射锥体神经元中错误表达Zfp312会诱导Tbr1（一种在深层神经元中富集的转录因子）的表达以及异位皮质下轴突投射的形成。因此，我们的结果表明转录因子Zfp312在皮质轴突投射和树突形态的层特异性及神经元亚型特异性模式形成中起关键作用。
Pyramidal neurons of the cerebal cortex display marked layer- and subtype-specific differences in their axonal projections and dendritic morphologies. Here we show that transcription factor Zfp312 is selectively expressed by layer V and VI subcortical projection pyramidal neurons and their progenitor cells. Knocking down Zfp312 with small interfering RNAs dramatically reduced the number of subcortical axonal projections from deep-layer pyramidal neurons and altered their dendritic morphology. In contrast, misexpression of Zfp312 in cortically projecting pyramidal neurons of layers 11 and III induced the expression of Tbr1, a transcription factor enriched in deep-layer neurons, and the formation of ectopic subcortical axonal projections. Thus, our results indicate that transcription factor Zfp312 plays a critical role in layer- and neuronal subtype-specific patterning of cortical axonal projections and dendritic morphologies.