Binding specificity of a baby hamster kidney lectin for H type I and II chains, polylactosamine glycans, and appropriately glycosylated forms of laminin and fibronectin.
Binding specificity of a baby hamster kidney lectin for H type I and II chains, polylactosamine glycans, and appropriately glycosylated forms of laminin and fibronectin.
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DOI：
10.1016/s0021-9258(19)50525-7
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发表时间：
1992-04
期刊：
影响因子：
--
通讯作者：
S. Sato;R. Hughes
中科院分区：
文献类型：
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作者：
S. Sato;R. Hughes
通过使用模型寡糖和糖肽抑制放射性标记的凝集素与脱唾液酸胎球蛋白-琼脂糖的结合，研究了来自幼仓鼠肾 (BHK) 细胞的 Mr = 30,000 凝集素 (CBP30) 的碳水化合物结合特异性。 CBP30 结合 I 型或 II 型 Gal beta(1—-3(4))GlcNAc 链，但不结合 Gal(beta 1—-3)GalNAc。直链聚乳糖胺结构或复合型支链聚糖的抑制效力与存在的 Gal(β 1—-3(4)) 单元的数量成比例地增加。末端半乳糖残基的岩藻糖基化或唾液酸化或通过(α1--3)-连接的半乳糖或N-乙酰半乳糖胺的进一步取代不影响结合，而倒数第二个N-乙酰葡糖胺残基的取代会大大降低结合。因此，A 型血、H I 型或 H II 型结构显示出高亲和力，而 Lex、Lea 和 Leb 结构则结合力较差。 CBP30 与鼠 Engelbreth-Holm-Swarm (EHS) 肿瘤层粘连蛋白和人羊水纤连蛋白结合，但不与人血浆纤连蛋白结合。结合涉及聚乳糖胺聚糖以及存在于 EHS 层粘连蛋白和羊水纤连蛋白中但不存在于血浆纤连蛋白中的三触角和四触角复合型聚糖。 EHS 层粘连蛋白的蛋白水解片段（E1X/Nd、P1、E8 和 E3）结合 CBP30，但只有片段 E8 支持 BHK 细胞的附着和扩散。 BHK 细胞与 EHS 层粘连蛋白或片段 E8 的粘附不受 CBP30 特异性抗体的干扰，但在相对高浓度（45 微克/ml）下，CBP30 抑制细胞在层粘连蛋白上的扩散和部分附着。
The carbohydrate binding specificity of Mr = 30,000 lectin (CBP30) from baby hamster kidney (BHK) cells has been studied by inhibition of binding of the radiolabeled lectin to asialofetuin-Sepharose using model oligosaccharides and glycopeptides. CBP30 binds type I or II Gal beta(1—-3(4))GlcNAc chains but not Gal(beta 1—-3)GalNAc. The inhibitory potency of straight chain polylactosamine structures or complex-type branched glycans is increased in proportion to the number of Gal(beta 1—-3(4)) units present. Fucosylation or sialylation of terminal galactose residues or further substitution by (alpha 1—-3)-linked galactose or N-acetylgalactosamine does not affect binding whereas substitution of the penultimate N-acetylglucosamine residue drastically reduces binding. Thus, blood group A, H type I or H type II structures, shows high affinity whereas Lex, Lea, and Leb structures bind poorly. CBP30 binds to murine Engelbreth-Holm-Swarm (EHS) tumor laminin and human amniotic fluid fibronectin but not human plasma fibronectin. Binding involves polylactosamine glycans as well as tri- and tetraantennary complex-type glycans present in EHS laminin and amniotic fluid fibronectin but absent in plasma fibronectin. Proteolytic fragments of EHS laminin (E1X/Nd, P1, E8, and E3) bind CBP30, but only fragment E8 supports attachment and spreading of BHK cells. BHK cell adhesion to EHS laminin or fragment E8 was not disturbed by CBP30-specific antibodies, but at relatively high concentrations (45 micrograms/ml) CBP30 inhibited spreading and partially attachment of cells on laminin.