Expression of the trk proto-oncogene is restricted to the sensory cranial and spinal ganglia of neural crest origin in mouse development.
Expression of the trk proto-oncogene is restricted to the sensory cranial and spinal ganglia of neural crest origin in mouse development.
复制标题
trk原癌基因的表达仅限于小鼠发育中神经嵴起源的感觉颅骨和脊髓神经节。
DOI：
10.1101/gad.4.5.683
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发表时间：
1990
影响因子：
10.5
通讯作者：
Parada,LF
中科院分区：
文献类型：
--
作者：
Martin-Zanca,D;Barbacid,M;Parada,LF
我们已经克隆并鉴定了人trk原癌基因的小鼠同源物，该基因是蛋白酪氨酸激酶（TK）受体基因家族的成员。在这里，我们提出了第一份报告的trk编码的mRNA物种在体内。在小鼠胚胎中的原位杂交分析揭示了一个显着的时间和空间调控的trk转录，表达局限于感觉颅（三叉神经，上级，颈静脉）和背根神经节（DRG）的神经嵴起源。最近的研究表明TK受体在胚胎发育过程中起调控作用。因此，小鼠和躯干的发育突变W和果蝇的发育突变sevenless代表了编码缺陷TK受体的基因。我们的数据表明，trk，一个与人类恶性肿瘤相关的基因，是一组神经嵴源性感觉神经元的特异性标志物，并且与该原癌基因可能在其表达的神经元的发育或表型中起重要作用的假设一致。
We have cloned and characterized the mouse homolog of the human trk proto-oncogene, a member of the protein tyrosine kinase (TK) receptor gene family. Here, we present the first report of a trk-encoded mRNA species in vivo. In situ hybridization analysis in the mouse embryo reveals a striking temporal and spatial regulation of trk transcription, with expression confined to the sensory cranial (trigeminal, superior, jugular) and dorsal root ganglia (DRG) of neural crest origin. Recent reports have shown that TK receptors can play regulatory roles in embryonic development. Thus, the developmental mutations W in mouse and torso and sevenless in Drosophila represent genes that code for defective TK receptors. Our data show that trk, a gene associated with malignancy in humans, is a specific marker for a set of neural crest-derived sensory neurons, and are consistent with the hypothesis that this proto-oncogene may have an important role in the development or phenotype of the neurons where it is expressed.