The dynamics of microbial partnerships in the coral Isopora palifera
The dynamics of microbial partnerships in the coral Isopora palifera
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DOI：
10.1038/ismej.2010.151
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发表时间：
2011-04-01
期刊：
影响因子：
11
通讯作者：
Tang, Sen-Lin
中科院分区：
文献类型：
--
作者：
Chen, Chung-Pin;Tseng, Ching-Hung;Tang, Sen-Lin
根据目前的报告，细菌和藻类共生体（共生藻属）是珊瑚全生体中的两个主要微生物伙伴，它们对环境的波动做出反应。然而，目前还没有证据表明这两个种群在季节性波动中存在任何直接的相互作用。在这项研究中，我们对 2008 年 2 月至 11 月每月采样后的三个独立珊瑚群落中的细菌和共生藻成分变化进行了现场观察。使用大规模并行焦磷酸测序，对超过 200 000 个细菌 V6 序列进行了分类，以构建细菌群落概况；此外，还通过实时PCR测定了共生藻进化枝C和D的相对组成和数量。结果表明，与珊瑚相关的细菌和共生藻群落在标记的珊瑚菌落中具有高度动态性和差异性，这表明宿主特异性的影响并不显着。与之前估计的其他珊瑚相比，与珊瑚相关的细菌群落更加多样化（香农指数高达 6.71），并且表现出快速的季节性变化。不同季节标记的珊瑚群落中共生藻 C 类群和 D 类群之间的种群比例有所不同；进化枝 D 在大多数样本中占主导地位。尽管没有检测到细菌和共生体之间的显着关联，但这项研究使用最新的分子方法，同时展示了珊瑚的这两种主要微生物伙伴的变化的更详细图片。 ISME 期刊 (2011) 5, 728-740； doi：10.1038/ismej.2010.151； 2010 年 10 月 21 日在线发布
Both bacteria and algal symbionts (genus Symbiodinium), the two major microbial partners in the coral holobiont, respond to fluctuations in the environment, according to current reports; however, little evidence yet indicates that both populations have any direct interaction with each other in seasonal fluctuation. In this study, we present field observations of a compositional change in bacteria and Symbiodinium in the coral Isopora palifera in three separate coral colonies following monthly sampling from February to November in 2008. Using massively parallel pyrosequencing, over 200 000 bacterial V6 sequences were classified to build the bacterial community profile; in addition, the relative composition and quantity of Symbiodinium clades C and D were determined by real-time PCR. The results showed that coral-associated bacterial and Symbiodinium communities were highly dynamic and dissimilar among the tagged coral colonies, suggesting that the effect of host specificity was insignificant. The coral-associated bacterial community was more diverse (Shannon index up to 6.71) than previous estimates in other corals and showed rapid seasonal changes. The population ratios between clade C and D groups of Symbiodinium varied in the tagged coral colonies through the different seasons; clade D dominated in most of the samples. Although significant association between bacteria and symbiont was not detected, this study presents a more detailed picture of changes in these two major microbial associates of the coral at the same time, using the latest molecular approaches. The ISME Journal (2011) 5, 728-740; doi:10.1038/ismej.2010.151; published online 21 October 2010