Dynamical Magnetic Response of Iron Oxide Nano articles Inside Live Cells
Dynamical Magnetic Response of Iron Oxide Nano articles Inside Live Cells
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DOI：
10.1021/acsnano.7b08995
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发表时间：
2018-03-01
期刊：
影响因子：
17.1
通讯作者：
Teran, Francisco J.
中科院分区：
文献类型：
--
作者：
Cabrera, David;Coene, Annelies;Teran, Francisco J.
暴露在交变磁场中的磁性纳米颗粒在癌症治疗中显示出巨大的潜力，可以作为磁性热疗介体。然而，当纳米颗粒位于细胞或组织内时，纳米颗粒的磁热效率显著降低，这是无法解释的。最近的研究表明，纳米粒子与细胞相互作用后的聚集和/或固定化作用的增强是可能的原因，尽管生物基质对磁性纳米粒子在交流磁场下的磁响应的影响仍然缺乏定量的描述。在这里，我们研究了细胞内化对氧化铁纳米颗粒(IONPs)动态磁响应的影响。两种磁芯大小(11和21微米)的交流磁测量和磁化率测量强调了细胞摄取后动态磁反应的差异，大小的影响更加明显。已经采用了两种方法来实验确定活细胞内磁性纳米颗粒的磁热损失，而不会危及它们的活性，以及磁性纳米结构在体外热疗研究中的适用性。我们的实验结果得到了理论计算的支持，表明细胞内IONP聚集的增强主要驱动细胞内化效应，而不是细胞内IONP的固定。了解纳米颗粒进入活细胞对其磁响应的影响，将有助于设计包含磁性纳米颗粒的纳米结构，其动态磁响应将在任何生物环境中保持不变，从而实现持续和可预测的体内加热效率。
Magnetic nanoparticles exposed to alternating magnetic fields have shown a great potential acting as magnetic hyperthermia mediators for cancer treatment. However, a dramatic and unexplained reduction of the nanoparticle magnetic heating efficiency has been evidenced when nanoparticles are located inside cells or tissues. Recent studies suggest the enhancement of nanoparticle clustering and/or immobilization after interaction with cells as possible causes, although a quantitative description of the influence of biological matrices on the magnetic response of magnetic nanoparticles under AC magnetic fields is still lacking. Here, we studied the effect of cell internalization on the dynamical magnetic response of iron oxide nanoparticles (IONPs). AC magnetometry and magnetic susceptibility measurements of two magnetic core sizes (11 and 21 tun) underscored differences in the dynamical magnetic response following cell uptake with effects more pronounced for larger sizes. Two methodologies have been employed for experimentally determining the magnetic heat losses of magnetic nanoparticles inside live cells without risking their viability as well as the suitability of magnetic nanostructures for in vitro hyperthermia studies. Our experimental results supported by theoretical calculations-reveal that the enhancement of intracellular IONP clustering mainly drives the cell internalization effects rather than intracellular IONP immobilization. Understanding the effects related to the nanoparticle transit into live cells on their magnetic response will allow the design of nanostructures containing magnetic nanoparticles whose dynamical magnetic response will remain invariable in any biological environments, allowing sustained and predictable in vivo heating efficiency.