Independent regulation of IgM, IgD, and Ia antigen expression in cultured immature B lymphocytes.
Independent regulation of IgM, IgD, and Ia antigen expression in cultured immature B lymphocytes.
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DOI：
10.1084/jem.163.4.938
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发表时间：
1986-04-01
影响因子：
15.3
通讯作者：
Jones, P P
中科院分区：
文献类型：
--
作者：
Dasch, J R;Jones, P P
对长期培养的骨髓细胞进行了多种B细胞和前B细胞标志物的表征。在根据Whitlock和Witte的方法建立的培养物中发现表达ThB、B-220和IgM的细胞。通过将来自骨髓的分选的前B细胞（ThB+，IgM-）与先前建立的骨髓粘附层一起培养来修改该培养系统。这些培养物开始生长，没有与惠特洛克培养物相关的滞后。这些培养的非贴壁细胞显示出高频率的IgM+细胞，但不表达IgD或Ia，我们将其称为未成熟B细胞。具有相似表型的细胞（IgM+、Ia-、IgD-）在幼年小鼠的脾脏中发现，但在成年小鼠的脾脏中未发现。佛波酯PMA诱导培养的未成熟B细胞表达IgD，但对Ia表达无影响。这表明H链RNA转录物的加工可能受到蛋白激酶C的影响。这些结果表明IgM、IgD和Ia的出现在长期培养的B系细胞中是独立控制的。
Long-term cultured bone marrow cells were characterized with respect to a number of B and pre-B cell markers. Cells expressing ThB, B-220, and IgM were found within cultures set up according to the procedure of Whitlock and Witte. This culture system was modified by placing sorted pre-B cells (ThB+, IgM-) from bone marrow in culture with previously- established bone marrow adherent layers. These cultures commenced growth without the lag associated with the Whitlock cultures. These cultured nonadherent cells show a high frequency of IgM+ cells, but do not express either IgD or Ia, and we refer to them as immature B cells. Cells with a similar phenotype (IgM+, Ia-, IgD-) are found within the spleens of young but not adult mice. The phorbol ester PMA induces expression of IgD on the cultured immature B cells, but has no effect on Ia expression. This suggests that the processing of H chain RNA transcripts may be affected by protein kinase C. These results demonstrate that the appearance of IgM, IgD, and Ia are independently controlled in long-term cultured B-lineage cells.