Enhancement of immune cellular agglutination by use of an avidin-biotin system.
Enhancement of immune cellular agglutination by use of an avidin-biotin system.
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通过使用抗生物素蛋白-生物素系统增强免疫细胞凝集。
DOI：
10.1093/clinchem/25.9.1572
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发表时间：
1979
影响因子：
9.3
通讯作者：
Roger W. Giese
中科院分区：
文献类型：
--
作者：
Sheila;M. Costello;Robert 1. Felix;Roger W. Giese
传统上，可以用抗球蛋白或第二抗体来增加凝集抗体或第一抗体的效价。我们使用了亲和素-生物素系统来代替抗球蛋白，类似地延长了初始抗细胞抗体的凝集。依次加入己酰胺生物素抗体、亲和素和扩张剂(己酰胺生物素大分子)，使红细胞凝集。被评价为扩充剂的大分子按效价递增顺序为：纤维蛋白原、白蛋白、琥珀酸化多赖氨酸和核糖核酸酶A。通过对比试验，我们发现抗球蛋白使抗体效价从2560提高到20480，亲和素-生物素工具使己胺双标抗体效价从2560提高到10240，加入己酰胺核糖核酸酶扩充剂后抗体效价从2560提高到81820。因此，非共价扩充剂增加了亲和素-生物素系统促进和替代抗体的能力。
Classically, one can increase the titer of an agglutinating or first antibody with an antiglobulin or second antibody. We have used an avidin-biotin system in place of antiglobulin to similarly extend the agglutination by an initial anticellular antibody. Erythrocytes were agglutinated by adding in succession, caproylamidobiotin-antibody, avidin, and extender (caproylamidobiotin-macromolecule). The macromolecules evaluated as extenders, in order of increasing potency, were fibrinogen, albumin, succinylated polylysine, and ribonuclease A. From comparative testing, we found that antiglobulin raised the titer of antibody from 2560 to 20,480, and the avidin-biotin tool raised the titer of caproylamidobiotin-antibody from 2560 to 10,240 without extender and to 81,820 with an extender of caproylamidobiotin-ribonuclease. Thus noncovalent extenders add to the capability of the avidin-biotin system to facilitate and substitute for an antibody.