A highly selective turn-on fluorescent probe for iron(II) to visualize labile iron in living cells
A highly selective turn-on fluorescent probe for iron(II) to visualize labile iron in living cells
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DOI：
10.1039/c2sc21649c
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发表时间：
2013-01-01
期刊：
影响因子：
8.4
通讯作者：
Nagasawa, Hideko
中科院分区：
文献类型：
--
作者：
Hirayama, Tasuku;Okuda, Kensuke;Nagasawa, Hideko
虽然不稳定的铁在活细胞中的各种生物过程中起着关键作用，但铁的生理和病理生理功能尚未得到充分探索，部分原因是缺乏可视化细胞内不稳定铁的方法。在这篇边缘文章中，我们提出了一种新的开启荧光探针（RhoNox-1），用于基于N-氧化物化学的Fe 2+的选择性检测。光谱研究结合DFT计算和电化学研究表明，RhoNox-1的荧光猝灭发生在生理条件下，这归因于叔胺N-氧化物取代的咕吨的激发态的非辐射失活，涉及扭曲的内部电荷转移（TICT）过程，部分原因是N-氧化物基团的光诱导电子转移（PET）。RhoNox-1通过选择性Fe 2+介导的N-氧化物基团脱氧，在Fe 2+负载细胞中显示出显著增强的荧光信号，并且还成功地检测了活细胞中的基础和内源性不稳定Fe 2+。
Although labile iron plays critical roles in diverse biological processes in living cells, the physiological and pathophysiological functions of iron have not been sufficiently explored, partially due to a lack of methods for visualizing intracellular labile iron. In this edge article, we present a novel turn-on fluorescent probe (RhoNox-1) for the selective detection of Fe2+ based on N-oxide chemistry. Spectroscopic studies combined with DFT calculations and electrochemical studies revealed that fluorescence quenching of RhoNox-1 occurred in physiological conditions, which was attributed to non-radiative deactivation of the excited state of tertiary amine N-oxide substituted xanthene involving a twisted internal charge transfer (TICT) process and partially due to photo-induced electron transfer (PET) from the N-oxide group. RhoNox-1 showed significant enhancement of the fluorescence signal in Fe2+-loaded cells via selective Fe2+-mediated deoxygenation of the N-oxide group and also successfully detected basal and endogenous labile Fe2+ in living cells.