Immunopathologic features of allergic contact dermatitis in humans: Participation of plasmacytoid dendritic cells in the pathogenesis of the disease?
Immunopathologic features of allergic contact dermatitis in humans: Participation of plasmacytoid dendritic cells in the pathogenesis of the disease?
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DOI：
10.1111/j.1523-1747.2003.12623.x
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发表时间：
2003-12-01
影响因子：
6.5
通讯作者：
Kopp, T
中科院分区：
文献类型：
--
作者：
Bangert, C;Friedl, J;Kopp, T
与我们对人类接触性超敏反应的致敏阶段的丰富知识相反，对协调效应阶段的细胞类型知之甚少。为了解决这个问题，我们表型分析了72小时表皮斑贴试验反应（n=10）和正常人皮肤（n=5）的各种白细胞分化抗原的存在活检。炎性浸润以CD 3（+）/CD 4（+）T细胞为主，约30%的细胞共表达CD 25和CTLA-4，这一表型与活化的效应或调节性T细胞一致。在我们寻找专职抗原呈递细胞的过程中，我们惊讶地发现不仅有大量的CD 1a（+）树突状细胞和CD 1c（+）树突状细胞，而且还有CD 123（+）、CD 45 RA（+）、BDCA-2（+）、CLA（+）和CD 62 L（+）浆细胞样树突状细胞。虽然在正常人皮肤中几乎不存在，但这些细胞在半抗原激发后6小时就可检测到，并且经常在CD 56（+）自然杀伤细胞附近发现，表明这些细胞类型之间存在功能性相互作用。对过敏性接触性皮炎中炎性浸润细胞组成及其动力学的详细了解，应成为研究湿疹反应持续和消退中起作用的免疫学和分子事件的基础。
Contrary to our abundant knowledge about the sensitization phase of human contact hypersensitivity, little is known about the cell types orchestrating the effector phase. In order to address this issue, we phenotypically analyzed biopsies from 72 h epicutaneous patch test reactions (n=10) and normal human skin (n=5) for the presence of various leukocyte differentiation antigens. The inflammatory infiltrate was dominated by CD3(+)/CD4(+) T cells with approximately 30% of the cells coexpressing CD25 and CTLA-4, a phenotype consistent with either activated effector or regulatory T cells. In our search for professional antigen-presenting cells, we were surprised to find not only sizeable numbers of CD1a(+) dendritic cells and CD1c(+) dendritic cells, but also of CD123(+), CD45RA(+), BDCA-2(+), CLA(+), and CD62L(+) plasmacytoid dendritic cells. Although virtually absent in normal human skin, these cells were detectable already 6 h after hapten challenge and were often found in close proximity to CD56(+) natural killer cells, indicative of a functional interaction between these cell types. The detailed knowledge of the cellular composition of the inflammatory infiltrate in allergic contact dermatitis and its kinetics should form the basis for the investigation of the immunologic and molecular events operative in the perpetuation and resolution of the eczematous response.