Rice histone deacetylase HDA704 positively regulates drought and salt tolerance by controlling stomatal aperture and density
Rice histone deacetylase HDA704 positively regulates drought and salt tolerance by controlling stomatal aperture and density
复制标题
水稻组蛋白脱乙酰酶HDA704通过控制气孔孔径和密度正向调节干旱和耐盐性
DOI：
10.1007/s00425-021-03729-7
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发表时间：
2021
期刊：
影响因子：
4.3
通讯作者：
Jun Duan
中科院分区：
文献类型：
--
作者：
Jinhui Zhao;Wei Zhang;Jaime A. Teixeira da Silva;Xuncheng Liu;Jun Duan
干旱和盐碱会损害作物生长，降低产量。气孔在植物抗非生物胁迫中起着重要作用。在本研究中，我们确定了RPD 3/HDA 1型组蛋白去乙酰化酶HDA 704作为一个积极的调节因子在干旱和耐盐性。HDA 704是干旱和盐胁迫诱导的。过量表达HDA 704基因可促进转基因水稻气孔关闭，减少气孔数量，减缓失水速率，从而提高水稻的耐旱性和耐盐性。相反，在转基因水稻中，HDA 704基因的敲除降低了气孔关闭，加快了水分流失速率，导致耐旱性和耐盐性下降。我们检测了水稻中正调控气孔开度和气孔密度的DST（Drought and Salt Tolerance）和ABIL 2（Abscisic Acid-insensitive Like 2）基因的转录表达。我们的研究结果表明，HDA 704直接结合DST和ABIL 2，通过组蛋白去乙酰化修饰抑制其表达。总的来说，这些发现揭示了HDA 704通过抑制DST和ABIL 2的表达来正向调节耐旱性和耐盐性。我们的研究结果提供了一个新的见解气孔调节植物的非生物胁迫耐受性的分子机制。
Drought and salinity can damage crop growth and reduce yield. Stomata play an important role in abiotic stress tolerance. In this study on rice, we identified the RPD3/HDA1-type histone deacetylase HDA704 as a positive regulatory factor in drought and salt tolerance. HDA704 was induced by drought and salt stresses. Overexpression of HDA704 in transgenic rice promoted stomatal closure, decreased the number of stomata and slowed down the rate of water loss, consequently resulting in increased drought and salt tolerance. By contrast, knockdown of HDA704 in transgenic rice decreased stomatal closure and accelerated the rate of water loss, leading to decrease drought and salt tolerance. We detected the transcript expression of DST (Drought and Salt Tolerance) and ABIL2 (Abscisic Acid-insensitive Like2), which positively regulate stomatal aperture and density in rice. Our results showed that HDA704 directly binds to DST and ABIL2, repressing their expression via histone deacetylation modification. Collectively, these findings reveal that HDA704 positively regulates drought and salt tolerance by repressing the expression of DST and ABIL2. Our findings provide a new insight into the molecular mechanisms of stomata-regulated abiotic stress tolerance of plants.