Dopamine D2 receptor plays a role in memory function:: implications of dopamine-acetylcholine interaction in the ventral hippocampus
Dopamine D2 receptor plays a role in memory function:: implications of dopamine-acetylcholine interaction in the ventral hippocampus
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DOI：
10.1007/s00213-005-0072-x
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发表时间：
2005-10-01
影响因子：
3.4
通讯作者：
Iguchi, A
中科院分区：
文献类型：
--
作者：
Fujishiro, H;Umegaki, H;Iguchi, A
理由：海马多巴胺能系统在记忆功能中的作用尚未阐明。目的：我们之前报道多巴胺D1受体（D2R）参与海马乙酰胆碱（ACh）释放的调节。在本研究中，我们进一步研究了海马中乙酰胆碱-多巴胺（DA）的相互作用及其在记忆功能中的作用。方法：实验1中，使用缺乏胆碱（Ch）的饲料喂养大鼠。我们使用被动回避任务检查了 D2R 拮抗剂雷氯必利对认知能力的影响。我们进一步进行体内微透析，以评估将 D2R 激动剂喹吡罗输注至腹侧海马对其释放乙酰胆碱的能力的影响。对于实验2，使用用正常食物喂养的大鼠。评估放射臂迷宫任务的表现，以检查海马注射 D2R 激动剂喹吡罗对毒蕈碱 ACh 拮抗剂东莨菪碱诱导的记忆损伤的影响。 结果：在实验 1 中，用 Ch 缺乏饲料喂养的大鼠表现出表现受损，表现为海马注射 D2R 拮抗剂后被动回避任务的保留试验潜伏期延长，并且在注射 D2R 拮抗剂后释放 ACh 的能力降低。 D2R 激动剂与正常饲料喂养的大鼠进行比较。在实验2中，腹腔注射东莨菪碱引起的记忆障碍通过腹侧海马注射D2R激动剂得到改善。结论：这些结果表明海马ACh-DA相互作用可能参与记忆加工。
Rationale: The role of the hippocampal dopaminergic system in mnemonic function has not been clarified yet.Objective: We previously reported that the dopamine D, receptor (D2R) is involved in the regulation of acethylcholin (ACh) release in the hippocampus. In this study, we further investigated ACh-dopamine (DA) interaction in the hippocampus and its involvement in mnemonic function.Methods: For experiment 1, rats fed with Cholin (Ch)-deficient chow were used. We examined the effects of D2R antagonist, raclopride, on cognitive performance using a passive avoidance task. We further can-led out in vivo microdialysis to assess the effect of infusion of D2R agonist, quinpirole, into the ventral hippocampus on its capacity to release ACh. For experiment 2, rats fed with normal chow were used. The performance of a radial arm maze task was assessed to examine the effects of hippocampal injection of D2R agonist, quinpirole, on memory impairment induced by scopolamine, a muscarinic ACh antagonist.Results: In experiment 1, rats fed with Ch-deficient chow showed impaired performances indicated by prolonged latency on retention trials of a passive avoidance task following the hippocampal injection of D2R antagonist, and showed reduced capacity to release ACh following the injection of D2R agonist compared with rats fed with normal chow. In experiment 2, memory impairment induced by the intraperitoneal injection of scopolamine was ameliorated by the injection of D2R agonist into the ventral hippocampus.Conclusion: These results indicate the possible involvement of hippocampal ACh-DA interaction in mnemonic processing.