Comparative analysis of the effect of glycyrrhizin diammonium and lithium chloride on infectious bronchitis virus infection in vitro
Comparative analysis of the effect of glycyrrhizin diammonium and lithium chloride on infectious bronchitis virus infection in vitro
复制标题
甘草酸二铵与氯化锂体外对传染性支气管炎病毒感染作用的比较分析
DOI：
10.1080/03079450902912184
复制
发表时间：
2009-01-01
期刊：
影响因子：
2.8
通讯作者：
Ren, Xiaofeng
中科院分区：
文献类型：
--
作者：
Li, Jing;Yin, Jiechao;Ren, Xiaofeng
采用细胞病变效应观察、噬斑减少实验和逆转录聚合酶链反应研究甘草酸二铵（GD）和氯化锂（LiCl）对禽传染性支气管炎病毒（IBV）细胞感染的影响。分别分析GD和LiCl对病毒、病毒感染细胞或经两种药物预处理的细胞的抗病毒作用。我们的结果表明，GD 具有直接的抗病毒活性，从而完全抑制细胞感染。用 GD 预处理细胞或感染后添加药物都没有减轻细胞感染，这证实了 GD 与 LiCl 不同，对 IBV 的抑制作用是病毒因子，而不是细胞因子。通过感染原代鸡胚肾细胞证实了两种药物的抑制作用。此外，感染细胞的凋亡与细胞病变效应呈正相关，可以通过有效的药物治疗来抑制。我们的数据表明，GD 和 LiCl 有可能通过不同的抗病毒机制在体外预防 IBV 感染。这些数据有助于有效使用抗病毒药物。
The effects of glycyrrhizin diammonium (GD) and lithium chloride (LiCl) on cell infection by avian infectious bronchitis virus (IBV) were investigated using cytopathic effect observation, plaque-reduction assay and reverse transcriptase-polymerase chain reaction. The anti-viral effect of GD and LiCl on virus, on virus-infected cells or on cells pre-treated by both drugs was analysed, respectively. Our results showed that GD had a direct antiviral activity, leading to complete inhibition of cell infection. The cell infection was not alleviated by either pre-treatment of cells with GD or addition of the drug post infection, confirming that the inhibitory effect of GD, unlike LiCl, on IBV is a viral factor, rather than a cellular factor. The inhibitory effect of both drugs was confirmed by infecting primary chicken embryo kidney cells. In addition, apoptosis of infected cells was positively related with cytopathic effect and could be inhibited by effective drug treatment. Our data indicate that GD and LiCl have potential to prevent IBV infection in vitro through different antiviral mechanisms. The data are helpful for using antivirals efficiently.