Quantitative dynamic evolution of physiological parameters of RBC by highly stable digital holographic microscopy
Quantitative dynamic evolution of physiological parameters of RBC by highly stable digital holographic microscopy
复制标题
DOI：
10.1016/j.optlaseng.2021.106887
复制
发表时间：
2022-04-01
影响因子：
4.6
通讯作者：
Awatsuji, Yasuhiro
中科院分区：
文献类型：
--
作者：
Kumar, Manoj;Matoba, Osamu;Awatsuji, Yasuhiro
数字全息显微镜（DHM）是一种功能强大的无标记成像工具，可提供样品的三维（3D）定量信息。在这项工作中，人类红细胞（RBC）的形态和定量参数的评价由一个新的单次共光路离轴数字全息显微系统的演示。所提出的系统是通过采用楔形板，镀银在其背面，到物体的光路产生两个光束：一个从前面和另一个从楔形板的背面。其中一束光束通过使用针孔进行空间滤波，以完全擦除其中的物体信息，并提供干净的参考光束，该参考光束与物体光束干涉，产生全息图。由于采用共光路结构，该系统具有较高的相位稳定性，因此更适合于小单元厚度波动的研究。此外，该系统简单、紧凑、较便宜且振动敏感性较低。本文报道了该系统对人红细胞的形态学和定量参数以及膜波动的测量结果。实验计算的参数的红细胞得到良好的协议与他们的正常生理范围。
Digital holographic microscopy (DHM) is a powerful label-free imaging tool that provides three-dimensional (3D) quantitative information of a specimen. In this work, the evaluation of morphological and quantitative parameters of human red blood cells (RBCs) by a new single-shot common-path off-axis digital holographic microscopic system is demonstrated. The proposed system is accomplished by employing a wedge plat, silver-coated at its back surface, into the object beam path generating two beams: one from the front surface and another from the back surface of the wedge plate. One of the beams is spatially filtered by using a pinhole to completely erase the object information from it and serving the clean reference beam, which on interfering with the object beam, creates the hologram. The proposed system, owing to common-path configuration, offers higher temporal phase stability, therefore, making it more suitable for the investigation of small cell thickness fluctuation. Moreover, the system is simple, compact, less expensive, and less vibration sensitive. The measurements of morphological and quantitative parameters, and membrane fluctuations of the human RBCs by the proposed system are reported. The experimentally calculated parameters of the RBC are obtained in good agreement with their normal physiological range.