Influence of a probiotic mixture on antibiotic induced microbiota disturbances
Influence of a probiotic mixture on antibiotic induced microbiota disturbances
复制标题
DOI：
10.3748/wjg.v20.i33.11878
复制
发表时间：
2014-09-07
影响因子：
4.3
通讯作者：
Ouwehand, Arthur C.
中科院分区：
文献类型：
--
作者：
Forssten, Sofia;Evans, Malkanthi;Ouwehand, Arthur C.
目的：研究益生菌摄入对抗生素暴露前后粪便微生物群的影响。方法：一项随机、双盲、安慰剂对照、平行组研究，使用两种益生菌组合[嗜酸乳杆菌（L. acidophilus） ATCC 700396和乳酸双歧杆菌（B. lactis） ATCC SD5220]在抗生素治疗（阿莫西林875和125 mg克拉维酸）期间和之后对健康成年人进行研究。通过实时聚合酶链反应研究了优势的粪便微生物群，以确定该益生菌制剂是否可以促进微生物群恢复到抗生素前的状态，并影响β -内酰胺耐药性的流行。采用问卷调查和布里斯托大便量表记录胃肠症状。结果：在第8天（抗生素治疗期结束），与安慰剂组相比，服用益生菌组合的受试者粪便中嗜酸乳杆菌ATCC 700396和乳酸杆菌的数量显著增加。此外，在第15天（益生菌治疗结束），服用益生菌组合的受试者粪便中嗜酸乳杆菌ATCC 700396和乳酸杆菌的数量明显高于服用安慰剂的受试者。在整个研究过程中，益生菌组的乳酸杆菌数量保持稳定，而XIV梭菌组在研究结束时更高，更接近基线水平；这与安慰剂组形成对比。抗生素暴露后β -内酰胺耐药性增加，两组间无差异。胃肠道症状通常是轻微的，治疗组之间没有差异，这与微生物群通常很小的变化有关。结论：食用益生菌组合主要导致制剂中所含菌种的粪便水平升高。（三）2014年百世登出版集团股份有限公司版权所有。
AIM: To study the effect of probiotic consumption on the faecal microbiota during and after antibiotic exposure.METHODS: A randomized, double-blind, placebo-controlled, parallel group study with a two species probiotic combination [Lactobacillus acidophilus (L. acidophilus) ATCC 700396 and Bifidobacterium lactis (B. lactis) ATCC SD5220] on healthy adults during and after antibiotic treatment (amoxicillin 875 and 125 mg clavulanate). The dominant faecal microbiota was studied by real time-polymerase chain reaction to determine if this probiotic preparation could facilitate restoring the microbiota to its pre-antibiotic state and influence the prevalence of beta-lactam resistance. Gastrointestinal symptoms were recorded by questionnaire and Bristol stool scale.RESULTS: Subjects on the probiotic combination had significantly higher faecal counts of L. acidophilus ATCC 700396 and B. lactis at day 8 (end of antibiotic treatment period) vs those on placebo. Furthermore, subjects on the probiotic combination had significantly higher faecal counts of L. acidophilus ATCC 700396 and B. lactis at Day 15 (end of probiotic treatment) vs those on placebo. Lactobacillus counts remained stable in the probiotic group over the course of the study, while Clostridium XIV group was higher at the end of the study and closer to baseline levels; this in contrast to the placebo group. Beta-lactam resistance in creased after antibiotic exposure and was not different between both treatment groups. Gastrointestinal symptoms were generally mild and did not differ between the treatment groups, which correlates with the generally small changes in the microbiota.CONCLUSION: Consumption of the probiotic combination mainly leads to an increase in the faecal levels of the species included in the preparation. (C) 2014 Baishideng Publishing Group Inc. All rights reserved.