Mitochondrial growth and DNA synthesis occur in the absence of nuclear DNA replication in fission yeast.
Mitochondrial growth and DNA synthesis occur in the absence of nuclear DNA replication in fission yeast.
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发表时间：
1990-11
影响因子：
4
通讯作者：
S. Sazer;Steven W. Sherwood
中科院分区：
文献类型：
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作者：
S. Sazer;Steven W. Sherwood
细胞生长和分裂需要细胞成分加倍，然后将它们平均分配到两个子细胞。在不断增长的群体中，线粒体与细胞体积的比率保持不变，每个细胞的线粒体基因组数量也保持不变。负责协调核和线粒体 DNA 合成以及平衡细胞和线粒体大小增加的机制尚不清楚。在裂殖酵母粟酒裂殖酵母的研究中，我们通过 Southern 印迹分析和流式细胞术对用各种 DNA 结合荧光染料染色的细胞进行细胞和线粒体 DNA 含量的定量，我们证明它们能够以不同的效率检测核和线粒体 DNA。在无法启动核DNA合成的条件细胞分裂周期突变体cdc10中，我们发现在没有核DNA复制的情况下线粒体DNA含量增加。这表明线粒体和核 DNA 合成并不是必然相关的。我们还表明，随着细胞伸长，线粒体尺寸增加并不需要线粒体 DNA 复制，尽管这会导致线粒体 DNA 与线粒体体积的比率下降。
Cell growth and division require the doubling of cellular constituents followed by their equal distribution to the two daughter cells. Within a growing population, the ratio of mitochondrial to cellular volume is maintained, as is the number of mitochondrial genomes per cell. The mechanisms responsible for coordinating nuclear and mitochondrial DNA synthesis, and for balancing increases in cell and mitochondrial size are not well understood. In studies of the fission yeast Schizosaccharomyces pombe we quantified cellular and mitochondrial DNA content by both Southern blot analysis and flow cytometry of cells stained with a variety of DNA-binding fluorochromes, which we show are able to detect nuclear and mitochondrial DNA with different efficiencies. In the conditional cell division cycle mutant cdc10, which is unable to initiate nuclear DNA synthesis, we found that there was an increase in the mitochondrial DNA content in the absence of nuclear DNA replication. This demonstrates that mitochondrial and nuclear DNA synthesis are not obligately linked. We also show that mitochondrial DNA replication is not required for the increase in mitochondrial size that occurs as cells elongate, although this results in a decrease in the ratio of mitochondrial DNA to mitochondrial volume.