A 10 residue motif at the C-terminus of the RNA pol II CTD is required for transcription, splicing and 3′ end processing
A 10 residue motif at the C-terminus of the RNA pol II CTD is required for transcription, splicing and 3′ end processing
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DOI：
10.1093/emboj/cdg396
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发表时间：
2003-08-15
期刊：
影响因子：
11.4
通讯作者：
Bentley, DL
中科院分区：
文献类型：
--
作者：
Fong, N;Bird, G;Bentley, DL
RNA聚合酶II C末端七肽重复结构域（CTD）对于mRNA前体的正常转录和共转录加工是必不可少的。哺乳动物CTD包含52个七肽，其共有序列YSPTSPS在整个真核生物中是保守的，随后是仅在脊椎动物中保守的10个氨基酸的C-末端序列。在这里，我们令人惊讶地表明，七肽重复不足以支持有效的转录，前mRNA加工或完整的细胞活力。除了七肽外，10个氨基酸的C-末端基序对于高水平转录、剪接和多聚腺苷酸位点切割是必需的。瞬时转染报告基因的高效mRNA合成需要C末端基序加上来自CTD N末端或C末端一半的16至25个七肽重复序列。27个共有七肽加上C-末端基序也支持有效的mRNA合成，但不支持细胞活力。
The RNA polymerase II C-terminal heptad repeat domain (CTD) is essential for normal transcription and co-transcriptional processing of mRNA precursors. The mammalian CTD comprises 52 heptads whose consensus, YSPTSPS, is conserved throughout eukaryotes, followed by a 10 amino acid C-terminal sequence that is conserved only among vertebrates. Here we show that surprisingly, the heptad repeats are not sufficient to support efficient transcription, pre-mRNA processing or full cell viability. In addition to the heptads, the 10 amino acid C-terminal motif is essential for high level transcription, splicing and poly(A) site cleavage. Efficient mRNA synthesis from a transiently transfected reporter gene required the C-terminal motif plus between 16 and 25 heptad repeats from either the N- or C-terminal half of the CTD. Twenty-seven consensus heptads plus the C-terminal motif also supported efficient mRNA synthesis but not cell viability.