Platelet activating factor activity in the phospholipids of bovine spermatozoa.
Platelet activating factor activity in the phospholipids of bovine spermatozoa.
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牛精子磷脂中的血小板激活因子活性。
DOI：
10.1095/biolreprod43.5.806
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发表时间：
1990
影响因子：
3.6
通讯作者：
Elrod,C
中科院分区：
文献类型：
--
作者：
Parks,JE;Hough,S;Elrod,C
血小板活化因子（PAF）已在几种哺乳动物的精子中检测到，并可影响精子活力和受精。由于牛精子含有高百分比的PAF合成所需的醚连接磷脂前体，因此进行了一项研究以确定牛精子磷脂的PAF活性。提取经洗涤、射出的公牛精子的总脂质，并通过薄层色谱法分离磷脂。根据马血小板释放的[3 H] 5-羟色胺，测定单个磷脂组分的PAF活性。在PAF组分（1.84 pmol/μmol总磷脂）和丝氨酸/肌醇（PS/PI）、胆碱（CP）、乙醇胺磷酸甘油酯（EP）和心磷脂（CA）组分中检测到PAF活性。CP组分的活性最高（8.05 pmol/μmol总磷脂）。PAF和中性脂质的不完全解析可能分别导致PS/PI和CA组分中的活性。非精子来源的磷脂不刺激血清素的释放。血小板活化纯化PAF和精子磷脂组分抑制受体拮抗剂SRI 63-675。这些结果表明，牛精子含有PAF和其他精子磷脂，特别是CP和EP，这是高甘油醚组分，能够受体介导的血小板活化。
Platelet activating factor (PAF) has been detected in sperm from several mammalian species and can affect sperm motility and fertilization. Because bovine sperm contain a high percentage of ether-linked phospholipid precursors required for PAF synthesis, a study was undertaken to determine the PAF activity of bovine sperm phospholipids. Total lipids of washed, ejaculated bull sperm were extracted, and phospholipids were fractionated by thin-layer chromatography. Individual phospholipid fractions were assayed for PAF activity on the basis of [3H]serotonin release from equine platelets. PAF activity was detected in the PAF fraction (1.84 pmol/μmol total phospholipid) and in serine/inositol (PS/PI), choline (CP), and ethanolamine phosphoglyceride (EP) and cardiolipin (CA) fractions. Activity was highest in the CP fraction (8.05 pmol/μmol total phospholipid). Incomplete resolution of PAF and neutral lipids may have contributed to the activity in the PS/PI and CA fractions, respectively. Phospholipids from nonsperm sources did not stimulate serotonin release. Platelet activation by purified PAF and by sperm phospholipid fractions was inhibited by the receptor antagonist SRI 63–675. These results indicate that bovine sperm contain PAF and that other sperm phospholipids, especially CP and EP, which are high in glycerylether components, are capable of receptor-mediated platelet activation.