Design of a Simplified Histopathologic Model for Gastrointestinal Inflammation in Dogs
Design of a Simplified Histopathologic Model for Gastrointestinal Inflammation in Dogs
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DOI：
10.1177/0300985813511123
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发表时间：
2014-09-01
影响因子：
2.4
通讯作者：
Day, M. J.
中科院分区：
文献类型：
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作者：
Jergens, A. E.;Evans, R. B.;Day, M. J.
即使使用世界小动物兽医协会（WSAVA）标准化标准，内镜胃肠道（GI）标本的诊断解释也存在显著的观察者间差异。卡方分析比较了病理学家对从原始WSAVA研究的253只动物中获得的内窥镜标本中炎症微结构特征的一致程度。病理学家之间的一致性模式分为广泛（3/4病理学家同意）、二分（2/4病理学家同意）或分歧（病理学家之间无一致性）。GI炎症的简化模型基于病理学家对组织病理学意义有广泛或最小分歧意见的参数。在该模型中，选择的参数如下：胃参数（上皮内淋巴细胞[IEL]、固有层[LP]浸润和粘膜纤维化）、十二指肠参数（绒毛萎缩、上皮损伤、IEL、隐窝变化和LP浸润）和结肠参数（上皮损伤、隐窝扩张、纤维化、LP浸润和杯状细胞耗竭）。使用该简化模型的初步数据显示，病理学家在定义犬GI炎症的存在和程度方面具有良好的相关性。
Significant interobserver variability in the diagnostic interpretation of endoscopic gastrointestinal (GI) specimens exists even with the use of World Small Animal Veterinary Association (WSAVA) standardization criteria. Chi-square analyses compared the extent of pathologists' agreement for microarchitectural features of inflammation in endoscopic specimens obtained from 253 animals of the original WSAVA study. Patterns of agreement between pathologists were classified as broad (3/4 pathologists agreed), dichotomous (2/4 pathologists agreed), or divergent (no agreement between pathologists). The simplified model for GI inflammation was based on those parameters for which the pathologists had either broad or minimally divergent opinions of histopathologic significance. In this model, the parameters chosen were as follows: gastric parameters (intraepithelial lymphocytes [IELs], lamina propria [LP] infiltrates, and mucosal fibrosis), duodenal parameters (villus atrophy, epithelial injury, IELs, crypt changes, and LP infiltrates), and colonic parameters (epithelial injury, crypt dilation, fibrosis, LP infiltrates, and goblet cell depletion). Preliminary data using this simplified model showed excellent correlation between pathologists in defining the presence and extent of GI inflammation in dogs.