Uukuniemi Phlebovirus assembly and secretion leave a functional imprint on the virion glycome.
Uukuniemi Phlebovirus assembly and secretion leave a functional imprint on the virion glycome.
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DOI：
10.1128/jvi.01662-14
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发表时间：
2014-09-01
影响因子：
5.4
通讯作者：
Bowden TA
中科院分区：
文献类型：
--
作者：
Crispin M;Harvey DJ;Bitto D;Halldorsson S;Bonomelli C;Edgeworth M;Scrivens JH;Huiskonen JT;Bowden TA
Uukuniemi病毒（UUKV）是研究布尼亚病毒科（Bunyaviridae）白蛉病毒属（Phlebovirus）的模式系统。我们报告UUKV糖组，揭示了Gn和Gc病毒粒子糖蛋白的差异加工。这两种糖蛋白显示聚-N-乙酰乳糖胺，与内侧高尔基体中的病毒体组装一致，而DC-SIGN依赖性细胞附着所需的寡甘露糖型聚糖主要在GC上。局部病毒体结构和病毒从细胞中排出的途径在静脉病毒糖组上留下功能印记。
Uukuniemi virus (UUKV) is a model system for investigating the genus Phlebovirus of the Bunyaviridae. We report the UUKV glycome, revealing differential processing of the Gn and Gc virion glycoproteins. Both glycoproteins display poly-N-acetyllactosamines, consistent with virion assembly in the medial Golgi apparatus, whereas oligomannose-type glycans required for DC-SIGN-dependent cellular attachment are predominant on Gc. Local virion structure and the route of viral egress from the cell leave a functional imprint on the phleboviral glycome.