A CpG island-encoded mechanism protects genes from premature transcription termination.
A CpG island-encoded mechanism protects genes from premature transcription termination.
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DOI：
10.1038/s41467-023-36236-2
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发表时间：
2023-02-09
影响因子：
16.6
通讯作者：
Klose, Robert J.
中科院分区：
文献类型：
--
作者：
Hughes, Amy L.;Szczurek, Aleksander T.;Kelley, Jessica R.;Lastuvkova, Anna;Turberfield, Anne H.;Dimitrova, Emilia;Blackledge, Neil P.;Klose, Robert J.
Transcription must be tightly controlled to regulate gene expression and development. However, our understanding of the molecular mechanisms that influence transcription and how these are coordinated in cells to ensure normal gene expression remains rudimentary. Here, by dissecting the function of the SET1 chromatin-modifying complexes that bind to CpG island-associated gene promoters, we discover that they play a specific and essential role in enabling the expression of low to moderately transcribed genes. Counterintuitively, this effect can occur independently of SET1 complex histone-modifying activity and instead relies on an interaction with the RNA Polymerase II-binding protein WDR82. Unexpectedly, we discover that SET1 complexes enable gene expression by antagonising premature transcription termination by the ZC3H4/WDR82 complex at CpG island-associated genes. In contrast, at extragenic sites of transcription, which typically lack CpG islands and SET1 complex occupancy, we show that the activity of ZC3H4/WDR82 is unopposed. Therefore, we reveal a gene regulatory mechanism whereby CpG islands are bound by a protein complex that specifically protects genic transcripts from premature termination, effectively distinguishing genic from extragenic transcription and enabling normal gene expression. Here the authors discover that SET1 complexes function as transcription anti-termination factors that bind to CpG islands and protect low to moderately transcribed genes from the pervasive termination activity of the ZC3H4 complex.
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影响因子：
16
作者：
Blackledge NP;Zhou JC;Tolstorukov MY;Farcas AM;Park PJ;Klose RJ
通讯作者：
Klose RJ
人 TFIIH 激酶 CDK7 调节转录相关的染色质修饰。
影响因子：
8.8
作者：
Ebmeier CC;Erickson B;Allen BL;Allen MA;Kim H;Fong N;Jacobsen JR;Liang K;Shilatifard A;Dowell RD;Old WM;Bentley DL;Taatjes DJ
通讯作者：
Taatjes DJ
影响因子：
4.6
作者：
Bledau, Anita S.;Schmidt, Kerstin;Anastassiadis, Konstantinos
通讯作者：
Anastassiadis, Konstantinos
影响因子：
10.5
作者：
Clouaire, Thomas;Webb, Shaun;Bird, Adrian
通讯作者：
Bird, Adrian
影响因子：
16
作者：
Eberl, H. Christian;Spruijt, Cornelia G.;Mann, Matthias
通讯作者：
Mann, Matthias