NUCLEOTIDE-SEQUENCE AND EXACT LOCALIZATION OF THE NEOMYCIN PHOSPHOTRANSFERASE GENE FROM TRANSPOSON TN5
NUCLEOTIDE-SEQUENCE AND EXACT LOCALIZATION OF THE NEOMYCIN PHOSPHOTRANSFERASE GENE FROM TRANSPOSON TN5
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DOI：
10.1016/0378-1119(82)90023-3
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发表时间：
1982-01-01
期刊：
影响因子：
3.5
通讯作者：
SCHALLER, H
中科院分区：
文献类型：
--
作者：
BECK, E;LUDWIG, G;SCHALLER, H
测定了含有新霉素磷酸转移酶基因（neo）的转座子Tn 5的独特区域的1200 bp [碱基对]的核苷酸序列，并通过792 bp的翻译阅读框中的缺失突变体鉴定了neo基因的位置。衍生的基因产物，氨基糖苷3“-磷酸转移酶（APH）II，由264个氨基酸残基组成，计算的MW为29，053。其氨基酸序列显示出与由转座子Tn 903编码的APH I型酶的序列同源性（Oka等人，1981年）。
The nucleotide sequence of 1200 bp [base pairs] from the unique region of transposon Tn5 containing the neomycin phosphotransferase gene (neo) was determined and the location of the neo gene was identified by deletion mutants in a translational reading frame of 792 bp. The derived gene product, an aminoglycoside 3''-phosphotransferase (APH) II, consists of 264 amino acid residues and has a calculated MW of 29,053. Its amino acid sequence shows sequence homologies to the APH type I enzyme coded for by transposon Tn903 (Oka et al., 1981).