Elimination of carbon catabolite repression in Klebsiella oxytoca for efficient 2,3-butanediol production from glucose-xylose mixtures
Elimination of carbon catabolite repression in Klebsiella oxytoca for efficient 2,3-butanediol production from glucose-xylose mixtures
复制标题
消除产酸克雷伯菌中碳分解代谢物的抑制，以从葡萄糖-木糖混合物中高效生产 2,3-丁二醇
DOI：
10.1007/s00253-010-2940-5
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发表时间：
2011-02-01
影响因子：
5
通讯作者：
Ouyang, Ping-Kai
中科院分区：
文献类型：
--
作者：
Ji, Xiao-Jun;Nie, Zhi-Kui;Ouyang, Ping-Kai
微生物对葡萄糖的偏好意味着对木质纤维素水解产物的不完全和/或缓慢利用，这是由称为碳分解代谢物阻遏（CCR）的调节机制引起的。在这项研究中，生产2，3-丁二醇（2，3-BD）的产酸克雷伯氏菌菌株被工程化以消除葡萄糖对木糖利用的抑制。编码突变型环磷酸腺苷（cAMP）受体蛋白CRP（in）的crp（in）基因，其功能不需要cAMP，在K.催产素该工程重组体可以同时利用葡萄糖和木糖的混合物，而不需要CCR。在葡萄糖和木糖混合物中，检查了工程化重组体的糖消耗和2，3-BD生产的概况，并显示葡萄糖和木糖可以同时消耗以产生2，3-BD。本研究提供了一种代谢工程策略，以实现高效利用来自木质纤维素生物质的糖混合物，用于使用肠道细菌生产生物基化学品。
Microbial preference for glucose implies incomplete and/or slow utilization of lignocellulose hydrolysates, which is caused by the regulatory mechanism named carbon catabolite repression (CCR). In this study, a 2,3-butanediol (2,3-BD) producing Klebsiella oxytoca strain was engineered to eliminate glucose repression of xylose utilization. The crp(in) gene, encoding the mutant cyclic adenosine monophosphate (cAMP) receptor protein CRP(in), which does not require cAMP for functioning, was characterized and overexpressed in K. oxytoca. The engineered recombinant could utilize a mixture of glucose and xylose simultaneously, without CCR. The profiles of sugar consumption and 2,3-BD production by the engineered recombinant, in glucose and xylose mixtures, were examined and showed that glucose and xylose could be consumed simultaneously to produce 2,3-BD. This study offers a metabolic engineering strategy to achieve highly efficient utilization of sugar mixtures derived from the lignocellulosic biomass for the production of bio-based chemicals using enteric bacteria.