Characterization of Electrochemical Activity of a Strain IS02-3 Phylogenetically Related to Aeromonas sp Isolated From a Glucose-Fed Microbial Fuel Cell
Characterization of Electrochemical Activity of a Strain IS02-3 Phylogenetically Related to Aeromonas sp Isolated From a Glucose-Fed Microbial Fuel Cell
复制标题
DOI：
10.1002/bit.22453
复制
发表时间：
2009-12-01
影响因子：
3.8
通讯作者：
Okahe, Satoshi
中科院分区：
文献类型：
--
作者：
Chung, Kyungmi;Okahe, Satoshi
采用16s rRNA法对葡萄糖供试的闭路和开路微生物燃料电池（MFCs）电极相关的微生物群落进行了分析和比较。通过比较发现，Gamaproteobacteria中的气单胞菌属（Aeromonas sp.）细菌构成了闭环MFC（收集电力）的主要种群，并被认为在当前发电中发挥重要作用。因此，我们试图从阳极生物膜中分离出优势菌，成功分离出一种与气单胞菌系统发育相关的Fe （III）还原菌，并将其命名为菌株ISO2-3。分离菌株ISO2-3能够生长并产生最大电流。0.24 A/m(2))通过葡萄糖或氢的氧化，电极作为唯一的电子受体。这种菌株可以发酵葡萄糖，但产生的电流更少。循环伏安法证实了ISO2-3具有电活性，并且可能通过膜相关化合物（很可能是c型细胞色素）将电子转移到电极上。这种机制需要与阳极表面紧密接触。扫描电镜显示，菌株ISO2-3在阳极表面形成多层生物膜，并产生锚状丝状附属物（很可能是菌毛），这可能促进电子在厚生物膜上的远程传递。Biotechnol。Bioeng。2009;104: 901 - 910。(C) 2009 Wiley期刊公司
The microbial communities associated with electrodes in closed and open circuit microbial fuel cells (MFCs) fed with glucose were analyzed by 16s rRNA approach and compared. The comparison revealed that bacteria affiliated with the Aeromonas sp. within the Gamaproteobacteria constituted the major population in the closed circuit MFC(harvesting electricity) and considered to play important roles in current generation. We, therefore attempted to isolated the dominant bacteria from the anode biofilm, succesfully isolated a Fe (III)-reducing bacterium phylogenetically related to Aeromonas sp. and designated as strain ISO2-3. The isolated strain ISO2-3 could grow and concomitantly produce current (max. 0.24 A/m(2)) via oxidation of glucose or hydrogen with an electrode serving as the sole electron acceptor. The strain could ferment glucose, but generate less electrical current. Cyclic voltammetry supported the strain ISO2-3 was electrically active and likely to transfer electrons to the electrode through membrane-associated compounds (most likely c-type cytochrome). This mechanism requires intimate contact with the anode surface. Scanning electron microscopy revealed that the strain ISO2-3 develeoped multilayer biofilms on anode surface and also produced anchor-like filamentous appendages, (most likely pili) that may promote long-range electron transport across the thick biofilm. Biotechnol. Bioeng. 2009; 104: 901-910. (C) 2009 Wiley Periodicals, Inc.