Skeletal tissue engineering - from in vitro studies to large animal models
Skeletal tissue engineering - from in vitro studies to large animal models
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DOI：
10.1016/s0142-9612(03)00492-7
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发表时间：
2004-04-01
期刊：
影响因子：
14
通讯作者：
Verdonschot, N
中科院分区：
文献类型：
--
作者：
Buma, P;Schreurs, W;Verdonschot, N
骨是一种具有强大再生潜力的组织。因此，骨组织工程的新策略应该只关注具有一定尺寸的不会自发愈合的缺损。在组织工程构建的发展中，许多变量可能发挥作用。如所用细胞的来源、支架的设计和力学性能以及生长因子的浓度和应用方式。研究骨组织工程新策略的模型应基于待修复的靶组织。然而，鉴于许多潜在的变量，如果组合使用，这些变量也可能相互作用，因此也非常需要相对简单的模型，其中变量可以在有限数量的动物中进行测试。此外，在受损的骨中，剩余健康骨的承载能力可能存在问题。在这种情况下，组织工程结构的一个重要先决条件是它们可以在负载条件下进行测试。特别是，后一个先决条件很难实现。因此，在动物实验之前，体外机械稳定性试验对于评价特定重建手术的机械后果非常有用。在组织工程构建体被引入临床试验之前，最后的测试应该在一个大型动物模型中进行，该模型尽可能接近和相关于特定的临床问题情况。在过去，我们实验室开发了一系列模型，这些模型对测试组织工程构建体非常有用。在本文中，我们专注于使用相对较新的简单的体外和体内模型，髋关节翻修手术，节段性骨缺损修复和肿瘤手术。(C)2003 Elsevier Ltd.保留所有权利。
Bone is a tissue with a strong regenerative potential. New strategies for tissue engineering of bone should therefore only focus on defects with a certain size that will not heal spontaneously. In the development of tissue-engineered constructs many variables may play a role. e.g. the source of the cells used, the design and mechanical properties of the scaffold and the concentration and mode of application of growth factor(s).Models for studying new strategies for tissue engineering of bone should be based on the target tissue to be restored. However, in light of the many potential variables, which may also interact if used in combination(s), there is also a large need for relatively simple models in which variables can be tested in a limited number of animals. Moreover, in compromised bone there may be a problem with the load-bearing capacity of the remaining healthy bone. In this light, an important prerequisite for tissue-engineering constructs is that they can be tested in loaded conditions. Particularly, this latter prerequisite is very difficult to achieve. Therefore, in vitro tests for mechanical stability are very useful for evaluating the mechanical consequences of a particular reconstruction procedure prior to the animal experiment. Before a tissue-engineered construct can be introduced into a clinical trial, a final test should be available in a large animal model that is as close and relevant to a particular problematic clinical situation as possible.In the past, a series of models were developed in our laboratory that are very useful for testing tissue-engineered constructs. In this paper, we focus on the use of relatively new simple in vitro and in vivo models for hip revision surgery, segmental bone defect restoration and tumour surgery. (C) 2003 Elsevier Ltd. All rights reserved.