Genetic Analysis of a Commercial Egg Laying Line Challenged With Newcastle Disease Virus
Genetic Analysis of a Commercial Egg Laying Line Challenged With Newcastle Disease Virus
复制标题
受新城疫病毒挑战的商业产蛋线的遗传分析
作者：
K. Rowland;A. Wolc;R. Gallardo;T. Kelly;Huaijun Zhou;J. Dekkers;S. Lamont
在低收入国家，鸡在日常生活中发挥着至关重要的作用。它们通过产蛋和产肉提供重要的蛋白质来源。新城疫由 1 型禽副粘病毒引起，被列为非洲和亚洲食腐鸡最具破坏性的疾病。感染强毒株的鸡群的高死亡率导致农村家庭膳食蛋白质和购买力的毁灭性损失。除疫苗接种外，提高鸡对新城疫病毒（NDV）的遗传抗性是低收入国家提高家禽生产的一个实际目标。由于对新城疫病毒的反应具有遗传控制的成分，因此可以通过选择性育种来影响它。将基因组信息添加到育种计划中可以增加每代的遗传进展量。在这项研究中，我们用新城疫病毒弱毒株挑战了商业产卵品系，测量了表型反应，收集了基因型以及与表型相关的基因型。收集的表型包括感染后 2 天和 6 天 (dpi) 时的病毒载量、攻击前和 10 dpi 的抗体水平以及攻击前和攻击后的生长率。鉴定出与 NDV 反应和/或生长相关的 6 个提示性 QTL，其中包括新的和已知的 QTL，证实了先前报道的与相关性状的关联。此外，之前的 RNA-seq 分析为位于或靠近已识别 QTL 的几个基因提供了支持。考虑到抗体和新城疫耐受性（疾病下生长）之间的负遗传相关趋势以及中度至高遗传力的估计，我们提供了证据表明这些新城疫反应性状可以通过选择性育种受到影响。生产在充满挑战的环境中表现良好的鸡最终将增加人类消费的优质蛋白质的供应。
In low income countries, chickens play a vital role in daily life. They provide a critical source of protein through egg production and meat. Newcastle disease, caused by avian paramyxovirus type 1, has been ranked as the most devastating disease for scavenging chickens in Africa and Asia. High mortality among flocks infected with velogenic strains leads to a devastating loss of dietary protein and buying power for rural households. Improving the genetic resistance of chickens to Newcastle Disease virus (NDV), in addition to vaccination, is a practical target for improvement of poultry production in low income countries. Because response to NDV has a component of genetic control, it can be influenced through selective breeding. Adding genomic information to a breeding program can increase the amount of genetic progress per generation. In this study, we challenged a commercial egg-laying line with a lentogenic strain of NDV, measured phenotypic responses, collected genotypes, and associated genotypes with phenotypes. Collected phenotypes included viral load at 2 and 6 days post-infection (dpi), antibody levels pre-challenge and 10 dpi, and growth rates pre- and post-challenge. Six suggestive QTL associated with response to NDV and/or growth were identified, including novel and known QTL confirming previously reported associations with related traits. Additionally, previous RNA-seq analysis provided support for several of the genes located in or near the identified QTL. Considering the trend of negative genetic correlation between antibody and Newcastle Disease tolerance (growth under disease) and estimates of moderate to high heritability, we provide evidence that these NDV response traits can be influenced through selective breeding. Producing chickens that perform favorably in challenging environments will ultimately increase the supply of quality protein for human consumption.
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影响因子：
9.8
作者：
Stevens, Elizabeth;Carss, Keren J.;Muntoni, Francesco
通讯作者：
Muntoni, Francesco
影响因子：
4
作者：
Kvainickas, Arunas;Orgaz, Ana Jimenez;Steinberg, Florian
通讯作者：
Steinberg, Florian
影响因子：
32.4
作者：
Heaton NS;Moshkina N;Fenouil R;Gardner TJ;Aguirre S;Shah PS;Zhao N;Manganaro L;Hultquist JF;Noel J;Sachs D;Hamilton J;Leon PE;Chawdury A;Tripathi S;Melegari C;Campisi L;Hai R;Metreveli G;Gamarnik AV;García-Sastre A;Greenbaum B;Simon V;Fernandez-Sesma A;Krogan NJ;Mulder LCF;van Bakel H;Tortorella D;Taunton J;Palese P;Marazzi I
通讯作者：
Marazzi I
影响因子：
4.6
作者：
Zhang H;Werner JP;García-Bustamante E;González-Rouco F;Wagner S;Zorita E;Fraedrich K;Jungclaus JH;Ljungqvist FC;Zhu X;Xoplaki E;Chen F;Duan J;Ge Q;Hao Z;Ivanov M;Schneider L;Talento S;Wang J;Yang B;Luterbacher J
通讯作者：
Luterbacher J