Embryonic Development of the Self-fertilizing Mangrove Killifish Kryptolebias marmoratus
Embryonic Development of the Self-fertilizing Mangrove Killifish Kryptolebias marmoratus
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DOI：
10.1002/dvdy.22668
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发表时间：
2011-07-01
影响因子：
2.5
通讯作者：
Kudoh, Tetsuhiro
中科院分区：
文献类型：
--
作者：
Mourabit, Sulayman;Edenbrow, Mathew;Kudoh, Tetsuhiro
红树林鳉是一种具有自我繁殖能力的脊椎动物，在许多生物学科中具有巨大的模式物种潜力。以前的研究已经定义了发育阶段，但缺乏各种胚胎结构的视觉表现。我们提供K的详细照片。marmoratus发展与修订的描述。一种改进的脱鳞方法，开发提供高分辨率的照片，除了显微注射技术，使细胞标记在蛋黄合胞体层。胚胎还用PTU（1-苯基2-硫脲）（一种黑素生成抑制剂）处理，以提供揭示发育后期内部结构的光学透明度。化学暴露（PTU和视黄酸）表明，K。marmoratus胚胎对化学品敏感，进一步说明了它们在发育生物学研究中的用途。我们的数据表明，K。marmoratus胚胎易于使用和操作，支持将这种雌雄同体脊椎动物用作胚胎学、进化、遗传学、环境和医学生物学中的强有力的比较模型系统。Developmental Dynamics 240：1694-1704，2011. (C)2011 Wiley-Liss，Inc.
The mangrove killifish, Kryptolebias marmoratus, is a self-fertilizing vertebrate offering vast potential as a model species in many biological disciplines. Previous studies have defined developmental stages but lacked visual representations of the various embryonic structures. We offer detailed photographic images of K. marmoratus development with revised descriptions. An improved dechorionation method was developed to provide high resolution photographs, in addition to a microinjection technique enabling cell marking in the yolk syncytial layer. Embryos were also treated with PTU (1-phenyl 2-thiourea), an inhibitor of melanogenesis, to provide optical transparency revealing internal structures in late stages of development. Chemical exposures (PTU and retinoic acid) demonstrated that K. marmoratus embryos were sensitive to chemicals, illustrating further their usefulness in developmental biology studies. Our data suggest that K. marmoratus embryos are easily used and manipulated, supporting the use of this hermaphroditic vertebrate as a strong comparative model system in embryology, evolution, genetics, environmental and medical biology. Developmental Dynamics 240: 1694-1704, 2011. (C) 2011 Wiley-Liss, Inc.