Bioluminescent Turn-On Probe for Sensing Hypochlorite in Vitro and in Tumors
Bioluminescent Turn-On Probe for Sensing Hypochlorite in Vitro and in Tumors
复制标题
用于体外和肿瘤中次氯酸盐传感的生物发光启动探针
DOI：
10.1021/acs.analchem.7b01103
复制
发表时间：
2017-06-06
影响因子：
7.4
通讯作者：
Liang, Gaolin
中科院分区：
文献类型：
--
作者：
Chen, Peiyao;Zheng, Zhen;Liang, Gaolin
Hypochlorite (ClO-) is one of the most important reactive oxygen species but using a BL probe for its-selective detection (or imaging) still remains challenging. Herein, We report a latent BL probe benzoylhydrazine luciferin (1) for highly selective detection of ClO- in vitro and imaging ClO- in living cells and tumors. In vitro tests indicated that 1 could be applied, for highly selective detection of ClO-, within the range,of 0-62.5 mu M with a limit of detection of 0.705 mu M. Using these unique features, of 1, we successfully applied it to image ClO- in living cells and tumors. We envision that probe 1 might be applied to elucidate the biological roles of ClO- in wider physiological and pathological processes in the near future.
