Identification of the first vancomycin intermediate-resistant Staphylococcus aureus (VISA) isolate from a hospital in Portugal
Identification of the first vancomycin intermediate-resistant Staphylococcus aureus (VISA) isolate from a hospital in Portugal
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DOI：
10.1089/mdr.2008.0816
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发表时间：
2008-03-01
影响因子：
2.6
通讯作者：
de Lencastre, H.
中科院分区：
文献类型：
--
作者：
Gardete, S.;Aires-De-Sousa, M.;de Lencastre, H.
2006年，从布拉加镇圣马科斯-布拉加镇圣马科斯-布拉加医院骨科病房住院的一名患者的手术伤口中分离到一株对万古霉素具有中等抗药性的耐甲氧西林金黄色葡萄球菌(MRSA)。结合脉冲场凝胶电泳(PFGE)、spA分型、多位点序列分型和葡萄球菌染色体盒mec分型等分子分型方法，将万古霉素中间耐药金黄色葡萄球菌VISA-Braga鉴定为流行的MRSA(EMRSA)-15克隆的衍生株，该克隆最近从葡萄牙几家医院分离频率增加。与具有相同遗传背景(包括PFGE亚型)的另一株EMRSA-15分离株相比，VISA-Braga分离株表现出相对较高的苯唑西林MIC，生长缓慢，溶血活性丧失，对万古霉素和达托霉素的耐药性增加，尽管这两种抗生素均未用于治疗。这里描述的Visa-Braga分离株似乎代表了葡萄牙一家医院发现的第一株对万古霉素敏感性降低的金黄色葡萄球菌。
A clinical isolate of methicillin-resistant Staphylococcus aureus (MRSA) with intermediate resistance to vancomycin (minimal inhibitory concentration [MIC] of 4 mu g/ml) was isolated in 2006 from a surgical wound of a patient hospitalized at the orthopedics ward of Hospital de Sao Marcos - Braga, in the town of Braga. A combination of molecular typing methods, including pulsed-field gel electrophoresis (PFGE), spa typing, multilocus sequence typing, and staphylococcal chromosomal cassette mec typing, identified the vancomycin intermediate-resistant S. aureus VISA-BRAGA as a derivative of the epidemic MRSA (EMRSA)-15 clone, which has been isolated with increasing frequency from several Portuguese hospitals recently. Compared to another EMRSA-15 isolate with the same genetic background (including PFGE subtype) the VISA-BRAGA isolate exhibited relatively high oxacillin MIC, slow growth, loss of hemolytic activity, and increased resistance to vancomycin and to daptomycin although neither of these two antibiotics was used in therapy. The VISA-BRAGA isolate described here appears to represent the first S. aureus with decreased susceptibility to vancomycin identified in a Portuguese hospital.