Biofilm-Forming Methicillin-Resistant Staphylococcus aureus Survive in Kupffer Cells and Exhibit High Virulence in Mice.
Biofilm-Forming Methicillin-Resistant Staphylococcus aureus Survive in Kupffer Cells and Exhibit High Virulence in Mice.
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DOI：
10.3390/toxins8070198
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发表时间：
2016-06-30
期刊：
影响因子：
4.2
通讯作者：
Jimi S
中科院分区：
文献类型：
--
作者：
Oyama T;Miyazaki M;Yoshimura M;Takata T;Ohjimi H;Jimi S
尽管金黄色葡萄球菌是正常体内菌群的一部分，但抗生素的大量使用导致了耐甲氧西林菌株（MRSA）的出现。 MRSA 可形成生物膜并引起留置异物感染、菌血症、软组织感染、心内膜炎和骨髓炎。使用体外测定，我们筛选了 173 种 MRSA 临床血液分离株，并选择了 20 种高生物膜形成物 (H-BF) 和低生物膜形成物 (L-BF)。将这些药物静脉注射给小鼠，研究小鼠的一般状况、细菌分布以及肝脏、肺、脾和肾中的生物膜。 MRSA 计数在肝脏中最高，尤其是在库普弗细胞内，其与细胞内生物膜相关的酸性多糖呈阳性。 24小时后，H-BF组小鼠的一般状况明显恶化。在肝脏中，H-BF组的细菌沉积和聚集以及生物膜形成点数量均显着多于L-BF组。 CFU分析显示，H-BF组的细菌在肝脏中存活了很长时间。这些结果表明，MRSA 的生物膜形成能力是细胞内持久存在的关键因素，可能导致慢性感染。
Although Staphylococcus aureus is part of the normal body flora, heavy usage of antibiotics has resulted in the emergence of methicillin-resistant strains (MRSA). MRSA can form biofilms and cause indwelling foreign body infections, bacteremia, soft tissue infections, endocarditis, and osteomyelitis. Using an in vitro assay, we screened 173 clinical blood isolates of MRSA and selected 20 high-biofilm formers (H-BF) and low-biofilm formers (L-BF). These were intravenously administered to mice and the general condition of mice, the distribution of bacteria, and biofilm in the liver, lung, spleen, and kidney were investigated. MRSA count was the highest in the liver, especially within Kupffer cells, which were positive for acid polysaccharides that are associated with intracellular biofilm. After 24 h, the general condition of the mice worsened significantly in the H-BF group. In the liver, bacterial deposition and aggregation and the biofilm-forming spot number were all significantly greater for H-BF group than for L-BF. CFU analysis revealed that bacteria in the H-BF group survived for long periods in the liver. These results indicate that the biofilm-forming ability of MRSA is a crucial factor for intracellular persistence, which could lead to chronic infections.