Establishment of a myeloid leukemia cell line, TRL-01, with MLL-ENL fusion gene
Establishment of a myeloid leukemia cell line, TRL-01, with MLL-ENL fusion gene
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DOI：
10.1016/j.cancergencyto.2005.09.008
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发表时间：
2006-08-01
影响因子：
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通讯作者：
Naoe, Tomoki
中科院分区：
文献类型：
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作者：
Ninomiya, Manabu;Abe, Akihiro;Naoe, Tomoki
我们通过异种移植到携带IL-2Rγ(C)-/-(NOG小鼠)的NOD/SCID小鼠体内，建立了t(11；19)的治疗相关急性髓系白血病细胞系。该细胞系TRL-01可以在小鼠之间连续移植，也可以在小鼠骨髓基质细胞系Hess-5上以黏附依赖的方式生长。Trl-01的免疫表型与原始白血病细胞相同：CD13、CD33、CDIa、CD18、CD29、CD49d、CD49e、CD54、CD62L和CD117阳性，CD3、CD4、CD8、CD19、CD34、CD41a、CD41b、CD135和髓过氧化物酶阴性。在原始样本和TRL-01中的易位(11；19)(q23；p13)分别产生了分别连接外显子6和外显子4的MLL-enl嵌合转录本，具有新的异构体。在TRL-01的培养中，加入GM-CSF、SCF和G-CSF以及与纤维连接蛋白包被的平板的黏附促进了短暂的增殖和存活，尽管它们不支持长期培养。皮下注射仅当Hess-5在同一部位联合注射时才会导致肿瘤形成。这些结果表明，TRL-01不仅是研究MLL-ENL白血病相关生物学的有用细胞系，而且是研究白血病与间质细胞间联系的有用细胞系。(C)2006 Elsevier Inc.保留所有权利。
We established a leukemia cell line derived from therapy-related acute myeloid leukemia with the t(11;19) by xenotransplantation into the NOD/SCID mouse with IL-2R gamma(c)-/- (NOG mouse). The cell line, TRL-01, could be serially transplanted from mouse to mouse and also grown in an adherence-dependent manner on a murine bone marrow stroma cell line, HESS-5. TRL-01 had the same immunophenotype as the original leukemia cells: positive for CD 13, CD33, CD I I a, CD 18, CD29, CD49d, CD49e, CD54, CD62L, and CD117, and negative for CD3, CD4, CD8, CD19, CD34, CD41a, CD41b, CD135, and myeloperoxidase. Translocation (11;19)(q23;p13) in both the original sample and TRL-01 generated MLL-ENL chimeric transcripts joining exon 6 and exon 4, respectively, which has a novel isoform. In cultures of TRL-01, addition of GM-CSF, SCF, and G-CSF and adhesion to fibronectin-coated plates promoted transient proliferation and survival, although they did not support long-term culture. Subcutaneous injection caused a tumor to form only when HESS-5 was coinjected at the same site. These results suggest that TRL-01 is a useful cell line for studying not only the leukemia-related biology of MLL-ENL but also the intercellular association between leukemia and stroma. (c) 2006 Elsevier Inc. All rights reserved.