Differential reorganisation of cutaneous elastic fibres: a comparison of the in vivo effects of broadband ultraviolet B versus solar simulated radiation.
Differential reorganisation of cutaneous elastic fibres: a comparison of the in vivo effects of broadband ultraviolet B versus solar simulated radiation.
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DOI：
10.1039/c7pp00412e
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发表时间：
2018-07-11
期刊：
影响因子：
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通讯作者：
Watson REB
中科院分区：
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作者：
Charoenchon N ;Rhodes LE ;Pilkington SM ;Farrar MD ;Watson REB
人体皮肤长期暴露在阳光下的紫外线辐射(UVR)会对皮肤的外观和功能产生负面影响。人类皮肤长期暴露在阳光下的紫外线辐射(UVR)会对其外观和功能产生负面影响，光老化的皮肤具有典型的坚韧、粗糙的外观，带有深深的皱纹。这些光损伤的临床特征被认为是由紫外线诱导的真皮细胞外基质，特别是弹性纤维系统重塑的结果。关于急性UVR照射对这一纤维系统的影响，特别是太阳模拟辐射(SSR)介导的影响，活体人体数据很少。我们研究了宽带UVB和SSR对人类真皮弹性纤维系统的不同影响，特别是微纤维成分纤维蛋白-1、纤维蛋白-2和纤维蛋白-5。健康白人成人(皮肤类型II-III型)接受3倍最小红斑量的宽带UVB(n=6)或SSR(n=6)照射在光保护的臀部皮肤上。照射后24小时和未照射的对照皮肤进行穿孔活检。总体而言，弹性纤维的组织学评估显示，与未照射的对照皮肤相比，宽带紫外线(P=0.004.0 5)或次同步辐射(P=0.0 4)照射的皮肤弹性纤维染色明显减少。UVB照射组皮肤乳头状真皮中纤维蛋白-1阳性微纤维染色明显减少(P=0.02)，而SSR照射组皮肤无明显变化。相反，纤维蛋白-5阳性微纤维的免疫组织化学显示，暴露于SSR的皮肤中的表达显著减少(P=0.04)，但在宽带UVB照射的皮肤中未见表达。无论是宽带UVB还是SSR照射后，纤维蛋白-2阳性微纤维均无明显变化。因此，宽带UVB和SSR对人体皮肤真皮弹性纤维网络的个别成分起着不同的调节作用。需要进一步的人体研究来探索这些发现背后的机制和潜在的光保护剂的影响。
Long-term exposure of human skin to ultraviolet radiation (UVR) in sunlight negatively impacts its appearance and function. Long-term exposure of human skin to ultraviolet radiation (UVR) in sunlight negatively impacts its appearance and function with photoaged skin having a characteristic leathery, rough appearance, with deep wrinkles. These clinical features of photodamage are thought to result from UVR-induced remodelling of the dermal extracellular matrix, particularly the elastic fibre system. There are few in vivo human data on the impact of acute UVR exposure on this fibre system and particularly solar-simulated radiation (SSR)-mediated effects. We examined the differential effect of broadband UVB and SSR on the human dermal elastic fibre system, and specifically the microfibrillar components fibrillin-1, fibulin-2 and fibulin-5. Healthy white Caucasian adults (skin type II–III) were recruited and irradiated with 3× their minimal erythema dose of broadband UVB (n = 6) or SSR (n = 6) on photoprotected buttock skin. Punch biopsies were taken 24 h after irradiation and from unirradiated control skin. Overall, histological assessment of elastic fibres revealed significantly less elastic fibre staining in broadband UVB (P = 0.004) or SSR (P = 0.04) irradiated skin compared to unirradiated control skin. Significantly less staining of fibrillin-1-positive microfibrils was also observed in the papillary dermis of UVB irradiated skin (P = 0.02) but not skin exposed to SSR. Conversely, immunohistochemistry for fibulin-5-positive microfibrils revealed significantly less expression in skin exposed to SSR (P = 0.04) but not to broadband UVB. There was no significant change in fibulin-2-positive microfibrils following either broadband UVB or SSR irradiation. Thus, broadband UVB and SSR mediate differential effects on individual components of the dermal elastic fibre network in human skin. Further human studies are required to explore the mechanisms underlying these findings and the impact of potential photoprotective agents.