FOXP3+regulatory and TIA-1+cytotoxic T lymphocytes in HIV-associated Hodgkin lymphoma
FOXP3+regulatory and TIA-1+cytotoxic T lymphocytes in HIV-associated Hodgkin lymphoma
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DOI：
10.1111/j.1440-1827.2011.02754.x
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发表时间：
2012-02-01
影响因子：
2.2
通讯作者：
Ohshima, Koichi
中科院分区：
文献类型：
--
作者：
Kiyasu, Junichi;Aoki, Ryosuke;Ohshima, Koichi
人类免疫缺陷病毒（HIV）感染CD 4+淋巴细胞，导致恶性淋巴瘤的发展，例如HIV相关霍奇金淋巴瘤（HIV-HL）。本研究旨在评估HIV-HL的炎症反应背景的细胞组成的差异。我们检查了浸润性T淋巴细胞，特别是调节性T细胞，细胞毒性细胞，EB病毒（EBV）相关抗原和HIV受体CCR 5。在所有HIV-HL病例中，除一例LMP-1染色呈阴性外，其余Hodgkin和Reed-Sternberg（HRS）细胞均呈EBER 1表达、LMP-1染色阳性、EBNA-2染色阴性。组织学检查结果显示，HIV-HL患者外周血中CD 8+、TIA-1+淋巴细胞的比例明显高于非HIV-HL患者（P < 0.05）。HIV-HL患者CD 4+、FOXP 3+淋巴细胞百分率显著低于非HIV-HL患者（P < 0.05），但仍存在。HIV-HL患者CCR 5+淋巴细胞百分率显著低于非HIV-HL患者（P < 0.05）。通常，据报道，在HIV相关非霍奇金淋巴瘤中很少检测到CD 4+和CCR 5+淋巴细胞，但CD 4+和/或FOXP 3+淋巴细胞的存在可能与HL的发病机制有关。此外，尽管额外的CD 8+淋巴细胞可能不是EBV-LMP特异性细胞毒性T细胞，但这些淋巴细胞也可能参与HIV-HL的发病机制。
Human immunodeficiency virus (HIV) infects CD4+ lymphocytes, leading to a development of malignant lymphomas, such as HIV-associated Hodgkin Lymphoma (HIV-HL). This study aimed to assess the differences in cellular composition of the inflammatory reactive background of HIV-HLs. We examined infiltrating T lymphocytes, specifically regulatory T cells, cytotoxic cells, Epstein-Barr virus (EBV) related antigens and HIV-receptor CCR5. In all HIV-HL cases, Hodgkin and Reed-Sternberg (HRS) cells showed EBER1 expression, LMP-1 staining positivity and EBNA-2 staining negativity, except for one case which showed LMP-1 staining negativity. Our histological findings indicate the percentage of CD8+, TIA-1+ lymphocytes was significantly higher in HIV-HL than in non-HIV-HL cases (P < 0.05). On the other hand, the percentage of CD4+, FOXP3+ lymphocytes was significantly lower in HIV-HL than in non-HIV-HL cases (P < 0.05) but present. The percentage of CCR5+ lymphocytes was significantly lower in HIV-HL than in non-HIV-HL cases (P < 0.05). Usually, CD4+ and CCR5+ lymphocytes are reported to be rarely detected in HIV-associated non-Hodgkin lymphomas, but the presence of CD4+ and/or FOXP3+ lymphocytes may be implicated in the pathogenesis of HL. In addition, although additional CD8+ lymphocytes are probably not EBV-LMP specific cytotoxic T-cells, these lymphocytes may also well be involved in the pathogenesis of HIV-HL.