Shape Matters: Gold Nanoparticle Shape Impacts the Biological Activity of siRNA Delivery.
Shape Matters: Gold Nanoparticle Shape Impacts the Biological Activity of siRNA Delivery.
复制标题
DOI：
10.1021/acs.bioconjchem.9b00004
复制
发表时间：
2019-02
影响因子：
4.7
通讯作者：
Erin Morgan;Dominik Wupperfeld;Demosthenes P. Morales;N. Reich
中科院分区：
文献类型：
--
作者：
Erin Morgan;Dominik Wupperfeld;Demosthenes P. Morales;N. Reich
等离子体共振纳米粒子提供了前所未有的时空控制，以释放不同的货物进入细胞。在这里，我们比较了中空金纳米壳、中空金纳米笼和带等离子体的金纳米棒的加载、释放和内化效率，以及转录后基因沉默的有效性，这些等离子体可以吸收800 nm的近红外光。与纳米笼和纳米棒相比，空心金纳米壳可以装载多达三倍的siRNA货物；然而，当暴露于脉冲800 nm激光激发时，纳米棒显示出最高的释放附着siRNA链的效率。在细胞处理中，所有颗粒都能有效地内化到HeLa细胞中，但纳米壳和纳米笼在处理72 h后显示出最高的GFP表达下调。这些结果为三种结构不同类型的金纳米颗粒作为siRNA载体的相对效率提供了新的见解，我们研究了可能影响其功效的不同参数。
Plasmon-resonant nanoparticles provide unprecedented spatiotemporal control over the release of diverse cargoes into cells. Here we compare the loading, release and internalization efficiencies, and effectiveness of post transcriptional gene silencing of hollow gold nanoshells, hollow gold nanocages, and gold nanorods with plasmons tuned to absorb near-infrared light at 800 nm. The hollow gold nanoshells can be loaded with up to three times more siRNA cargo compared to nanocages and nanorods; however, nanorods exhibit the highest efficiency of release of attached siRNA strands when exposed to pulsed 800 nm laser excitation. In cellular treatments, all particles demonstrated efficient internalization into HeLa cells, but the nanoshells and nanocages display the highest downregulation of GFP expression 72 h after treatment. These results provide novel insights into the relative efficiencies of three structurally distinct types of gold nanoparticles as siRNA carriers and we examine different parameters that may influence their efficacy.