Experimental and Theoretical Evidence for Bidirectional Signaling via Core Planar Polarity Protein Complexes in Drosophila.
Experimental and Theoretical Evidence for Bidirectional Signaling via Core Planar Polarity Protein Complexes in Drosophila.
复制标题
果蝇中通过核心平面极性蛋白复合物进行双向信号传导的实验和理论证据。
DOI：
10.1016/j.isci.2019.06.021
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发表时间：
2019
期刊：
影响因子：
5.8
通讯作者：
Fisher KH
中科院分区：
文献类型：
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作者：
Fisher KH
在发育中的组织中，细胞片层变成平面极化的，使得细胞行为能够协调。有人提出，细胞间极性信息的“信号传导”可能在分子Frizzled（Fz）和货车Gogh（旺）之间双向或单向发生。使用计算建模，我们发现，双向和单向信号模型再现已知的非自主表型来自补丁的突变组织的关键分子，但预测不同的表型从双突变组织，这在以前给出了相互矛盾的实验结果。此外，我们重新研究了果蝇翼的实验表型，得出的结论是，信号很可能是双向的。我们的模型表明，双向信号可以介导，无论是间接通过双向反馈之间的不对称细胞间蛋白质复合物或直接通过不同的亲和力蛋白质结合在细胞间复合物，建议未来的研究途径。我们的研究结果提供了深入了解阿曲他克林细胞信号传导机制以及组织规模的属性如何从单个细胞行为中出现。
In developing tissues, sheets of cells become planar polarized, enabling coordination of cell behaviors. It has been suggested that "signaling" of polarity information between cells may occur either bidirectionally or monodirectionally between the molecules Frizzled (Fz) and Van Gogh (Vang). Using computational modeling we find that both bidirectional and monodirectional signaling models reproduce known non-autonomous phenotypes derived from patches of mutant tissue of key molecules but predict different phenotypes from double mutant tissue, which have previously given conflicting experimental results. Furthermore, we re-examine experimental phenotypes in theDrosophilawing, concluding that signaling is most likely bidirectional. Our modeling suggests that bidirectional signaling can be mediated eitherindirectlyvia bidirectional feedbacks between asymmetric intercellular protein complexes ordirectlyvia different affinities for protein binding in intercellular complexes, suggesting future avenues for investigation. Our findings offer insight into mechanisms of juxtacrine cell signaling and how tissue-scale properties emerge from individual cell behaviors.