A novel microscope system for time-lapse observation of corneal cells in a living mouse
A novel microscope system for time-lapse observation of corneal cells in a living mouse
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DOI：
10.1016/s0014-4835(03)00210-0
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发表时间：
2004-03-01
影响因子：
3.4
通讯作者：
Nagasaki, T
中科院分区：
文献类型：
--
作者：
Maurice, DM;Zhao, J;Nagasaki, T
提出了一种新颖的显微镜系统，用于观察活体小鼠的角膜细胞。它能够在不同时间跟踪角膜各层中的单个细胞，从而生成延时记录。该系统由三个主要组件组成：用于可视化角膜细胞的直立荧光显微镜、用于固定动物和眼睛的小鼠固定装置以及一组无需重新聚焦即可观察大面积角膜表面的万向节。借助角膜中的基准标记，可以在不同时间观察到相同的细胞，从而可以在自然和实验条件下确定它们的位置变化。该技术应可用于研究正常和患病角膜的细胞运动，包括研究受伤或手术后的伤口愈合。 (C) 2003 Elsevier Ltd. 保留所有权利。
A novel microscope system is presented for observation of corneal cells in a living mouse. It enables tracking of individual cells in all layers of the cornea at various times, thus allowing the generation of time-lapse recordings. The system consists of three major components: an upright fluorescence microscope for visualization of corneal cells, a mouse-holding unit for immobilization of the animal and the eye, and a set of gimbals which permit observation of a wide area of corneal Surface Without refocusing. The same cells Could be observed at different times with the help of fiducial marks in the cornea, allowing their changes in position to be determined under natural and experimental conditions. This technique should prove useful in investigation of the cell movement in normal and diseased corneas, including the Study of wound healing after an injury or surgery. (C) 2003 Elsevier Ltd. All rights reserved.