Entry and distribution of microglial cells in human embryonic and fetal cerebral cortex
Entry and distribution of microglial cells in human embryonic and fetal cerebral cortex
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DOI：
10.1097/nen.0b013e3180517b46
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发表时间：
2007-05-01
影响因子：
3.2
通讯作者：
Verney, Catherine
中科院分区：
文献类型：
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作者：
Monier, Anne;Adle-Biassette, Homa;Verney, Catherine
在妊娠的前两个三个月，小胶质细胞按照特定的时空模式穿透并散布在人的皮质灰质和白质中。进入的途径在数量和质量上都与间脑中发现的不同。从4.5周开始，以Iba1、CD68、CD45和MHC-II等不同抗体为特征的阿米巴样小胶质细胞从脑室腔和软脑膜进入脑壁。迁移主要是向未成熟的白质、板下层和皮质板的径向和切向迁移，而软膜细胞填充在预期的I层。实质内的血管进入路径只有在12周时才能检测到。有趣的是，小胶质细胞聚集在限制的板层带中，特别是在妊娠19周至24周的放射冠纤维之间，在未成熟的白质中向尾部延伸，达到视觉辐射。这种增殖的MIBI阳性小胶质细胞(如MIB1-Iba1双重免疫标记所示)位于早产儿脑白质损伤的部位。未成熟白质和灰质中小胶质细胞的时空组织表明，这些细胞可能在发育过程中发挥积极作用，并在发育中的脑损伤中发挥作用。
Microglial cells penetrate into and scatter throughout the human cortical grey and white matter according to a specific spatiotemporal pattern during the first 2 trimesters of gestation. Routes of entry were quantitatively and qualitatively different from those identified in the diencephalon. Starting at 4.5 gestational weeks, amoeboid microglial cells, characterized by different antibodies as Iba1, CD68, CD45, and MHC-II, entered the cerebral wall from the ventricular lumen and the leptomeninges. Migration was mainly radial and tangential toward the immature white matter, subplate layer, and cortical plate, whereas pial cells populated the prospective layer I. The intraparenchymal vascular route of entry was detectable only from 12 gestational weeks. Interestingly, microglial cells accumulated in restricted laminar bands particularly at 19, to 24 gestational weeks among the corona radiata fibers rostrally, extending caudally in the immature white matter to reach the visual radiations. This accumulation of proliferating MIBI-positive microglia (as shown by MIB1-Iba1 double immunolabeling) was located at the site of white matter injury in premature neonates. The spatiotemporal organization of microglia in the immature white and grey matter suggests that these cells may play active roles in developmental processes and in injury to the developing brain.