High-throughput 3D cell invasion chip enables accurate cancer metastatic assays.
High-throughput 3D cell invasion chip enables accurate cancer metastatic assays.
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DOI：
10.1021/ja5072114
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发表时间：
2014-10-29
影响因子：
15
通讯作者：
Qin, Lidong
中科院分区：
文献类型：
--
作者：
Zhang, Yuanqing;Zhou, Ledu;Qin, Lidong
趋化性是细胞响应细胞外化学梯度而引导细胞迁移和侵袭的现象。肿瘤细胞以及肿瘤相关炎症细胞和基质细胞的趋化性由趋化因子、趋化因子受体、生长因子和生长因子受体介导。目前用于研究趋化驱动的细胞侵袭和转移的技术利用二维迁移测定，涉及对载玻片或塑料表面上的肿瘤细胞进行成像和分析，这需要大量细胞，并且通常缺乏对垂直细胞运动的实时监测和系统控制的趋化梯度，导致与临床研究和动物模型的结果相矛盾的结果。我们通过开发具有 4000 个超小型孔的高通量微型设备来解决这些挑战，以监测各种细胞密度下的实时三维细胞侵袭，并筛选抑制细胞侵袭并可能预防转移性恶性肿瘤的药物。此外，这种微型设备可以在同一芯片上为两种类型的细胞产生相反的梯度，从而构建一个具有顺序变化组件的受控系统，以研究基础细胞和免疫细胞的环境影响。
Chemotaxis is the phenomenon by which the migration and invasion of cells is directed in response to an extracellular chemical gradient. Chemotaxis of tumor cells and tumor-associated inflammatory and stromal cells is mediated by chemokines, chemokine receptors, growth factors, and growth factor receptors. Current techniques used to study chemotactic driven cell invasion and metastasis utilize two-dimensional migration assays involving imaging and analyzing tumor cells on glass slides or plastic surfaces, which requires large numbers of cells and often lacks real-time monitoring of vertical cell movement and systematically controlled chemotactic gradients, leading to contradictory results compared to those from clinical investigations and animal models. We addressed such challenges by developing a high-throughput microdevice with 4000 ultraminiaturized wells to monitor real-time, three-dimensional cell invasion over a wide range of cell densities and also screen drugs that inhibit cell invasion and potentially prevent metastatic malignancy. Additionally, this microdevice generates opposing gradients for two types of cells on the same chip, which builds a controlled system with sequentially changing components to study environmental effects from basal and immune cells.
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影响因子：
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