Identification of a microsatellite instability phenotype in meningiomas.
Identification of a microsatellite instability phenotype in meningiomas.
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DOI：
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发表时间：
1994-12
期刊：
影响因子：
11.2
通讯作者：
M. Pykett;M. Murphy;P. Harnish;D. George
中科院分区：
文献类型：
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作者：
M. Pykett;M. Murphy;P. Harnish;D. George
为了更好地了解脑膜瘤肿瘤发生的分子机制，我们以前利用消减杂交方法来鉴定这些常见脑肿瘤中表达或结构发生改变的基因。在这里，我们表明，CA二核苷酸重复元件存在于一个互补的DNA分离这种方法已经经历了在脑膜瘤细胞系的大小收缩。这一初步观察的扩展揭示了广泛的遗传变异影响简单重复序列在这个和其他脑膜瘤。这些数据表明，遗传不稳定性可能在脑膜瘤的病因学中起着以前未被认识到的作用。
To better understand the molecular mechanisms responsible for meningioma tumorigenesis we previously utilized subtractive hybridization protocols to identify genes the expression or structure of which is altered in these common brain tumors. Here we show that a CA dinucleotide repeat element present in one complementary DNA isolated by this approach has undergone a contraction in size in a meningioma cell line. Extension of this initial observation has revealed widespread genetic alterations affecting simple repeat sequences in this and other meningiomas. These data indicate that genetic instability may play a previously unrecognized role in the etiology of meningiomas.