The Ccr4-Not Complex: Architecture and Structural Insights
The Ccr4-Not Complex: Architecture and Structural Insights
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DOI：
10.1007/978-3-319-46503-6_13
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发表时间：
2017-01-01
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影响因子：
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通讯作者：
Panasenko, Olesya O.
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文献类型：
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作者：
Collart, Martine A.;Panasenko, Olesya O.
Ccr 4-Not复合物是一种重要的多亚基蛋白复合物，在真核mRNA代谢中起着重要作用，并具有影响真核基因表达的多种不同作用。它有一个保守的核心，三个不是蛋白，Ccr 4蛋白，和两个Ccr 4相关因子，Caf 1和Caf 40。第四种Not蛋白Not 4是保守的，但只是酵母中复合物的稳定亚基。某些亚基在进化过程中被复制，具有功能分歧，例如酵母中的Not 3和人类中的Ccr 4或Caf 1。然而，该复合物仅包括每个蛋白质的一个同源物。此外，物种特异性亚基是复合物的一部分，例如酵母中的Caf 130或人类中的Not 10和Not 11。两个保守的催化功能与复合物，去腺苷化和泛素化。该复合物采用L形结构，其中不同的模块与大的Not 1支架蛋白结合。在本章中，我们将总结我们目前对复合体的结构及其组成部分的结构的了解。
The Ccr4-Not complex is an essential multi-subunit protein complex that plays a fundamental role in eukaryotic mRNA metabolism and has a multitude of different roles that impact eukaryotic gene expression. It has a conserved core of three Not proteins, the Ccr4 protein, and two Ccr4 associated factors, Caf1 and Caf40. A fourth Not protein, Not4, is conserved, but is only a stable subunit of the complex in yeast. Certain subunits have been duplicated during evolution, with functional divergence, such as Not3 in yeast, and Ccr4 or Caf1 in human. However the complex includes only one homolog for each protein. In addition, species-specific subunits are part of the complex, such as Caf130 in yeast or Not10 and Not11 in human. Two conserved catalytic functions are associated with the complex, deadenylation and ubiquitination. The complex adopts an L-shaped structure, in which different modules are bound to a large Not1 scaffold protein. In this chapter we will summarize our current knowledge of the architecture of the complex and of the structure of its constituents.