BARLEY TELOMERES ARE ASSOCIATED WITH 2 DIFFERENT TYPES OF SATELLITE DNA-SEQUENCES
BARLEY TELOMERES ARE ASSOCIATED WITH 2 DIFFERENT TYPES OF SATELLITE DNA-SEQUENCES
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DOI：
10.1007/bf00713070
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发表时间：
1995-08-01
影响因子：
2.6
通讯作者：
GANAL, MW
中科院分区：
文献类型：
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作者：
BRANDES, A;RODER, MS;GANAL, MW
通过大麦中的荧光原位杂交 (FISH) 和脉冲场凝胶电泳 (PFGE) 分析了两种不同类型卫星 DNA 序列的基因组组织。除2号和7号染色体长臂外，所有染色体末端均检测到卫星HvT01。在标准核型上，除7号染色体长臂外的所有染色体末端以及两个间质位点均发现不相关的卫星pAS1，这两个位点均位于4号染色体长臂上。 Southern 杂交和原位杂交进一步表明 pAS1 也散布在大麦基因组中。对于大多数染色体末端，HvT01 和 pAS1 的线性顺序无法通过原位杂交确定，除了在 2 号和 6 号染色体的短臂处，其中 HvT01 比 pAS1 更远。 PFGE 分析证实了这一点，HvT01 经常与端粒重复序列相关，但与 pAS1 无关。此外，我们发现 HvT01 出现在大小高达 1000 kb 的簇中，而 pAS1 簇的最大大小为 500 kb。序列比较表明，两颗卫星完全不相关，并且 G + C 含量差异很大。
The genomic organization of two different types of satellite DNA sequences was analysed by means of fluorescence in situ hybridization (FISH) and pulsed-field gel electrophoresis (PFGE) in barley. Satellite HvT01 was detected at all chromosome ends except the long arms of chromosomes 2 and 7. The unrelated satellite pAS1 was found at all chromosome ends except the long arm of chromosome 7 and at two interstitial sites, both located on the long arm of chromosome 4 on the standard karyotype. Southern and in situ hybridizations further indicate that pAS1 also occurs interspersed in the barley genome. For most chromosome ends, the linear order of HvT01 and pAS1 could not be determined by in situ hybridization except at the short arms of chromosomes 2 and 6, where HvT01 is more distal than pAS1. This is confirmed by PFGE analysis, HvT01 being frequently associated with the telomeric repeat but not pAS1. Furthermore, we found that HvT01 occurred in clusters up to 1000 kb in size, whereas the pAS1 cluster had a maximum size of 500 kb. Sequence comparison revealed that both satellites are completely unrelated and differ considerably in their G + C contents.