Generation and Evaluation of Recombinant Baculovirus Coexpressing GP5 and M Proteins of Porcine Reproductive and Respiratory Syndrome Virus Type 1
Generation and Evaluation of Recombinant Baculovirus Coexpressing GP5 and M Proteins of Porcine Reproductive and Respiratory Syndrome Virus Type 1
复制标题
共表达猪繁殖与呼吸综合征病毒 1 型 GP5 和 M 蛋白的重组杆状病毒的产生和评价。
DOI：
10.1089/vim.2021.0018
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发表时间：
2021-12-01
期刊：
影响因子：
2.2
通讯作者：
Li，Chang
中科院分区：
文献类型：
--
作者：
Xu，Wang;Du，Shouwen;Li，Chang
猪繁殖与呼吸综合征病毒（PRRSV）是猪繁殖与呼吸综合征的病原体，猪繁殖与呼吸综合征是对养猪业最具经济破坏性的疾病之一。然而，灭活疫苗和改性减毒活疫苗是否有效控制疾病仍存在争议。虽然有几个研究小组开发了PRRSV病毒样颗粒（vlp）作为PRRSV的疫苗，但所有这些基于vlp的疫苗都针对PRRSV-2，而不是PRRSV-1或两者。因此，迫切需要研制抗PRRSV-1的VLPs。在本研究中，我们通过Bac-to-Bac®杆状病毒表达系统拯救了表达PRRSV-1的GP5和M蛋白的重组杆状病毒。随后，在重组杆状病毒感染的高掌虫细胞中高效地获得了PRRSV VLP。此外，PRRSV VLP和PRRSV VLP+A5可通过鼻内免疫有效触发特异性体液免疫反应和B细胞免疫反应。PRRSV VLP与A5佐剂联合使用可提高免疫应答水平。本研究产生的PRRSV-1 vlp具有更大的潜力用于疫苗开发以控制PRRSV-1感染。
Porcine reproductive and respiratory syndrome virus (PRRSV) is the pathogen of the porcine reproductive and respiratory syndrome, which is one of the most economically devastating diseases of the swine industry. However, whether the inactivated vaccine and modified live attenuated vaccines are effective in disease control is still controversial. Although several groups developed PRRSV virus-like particles (VLPs) as a vaccine against PRRSV, all these VLP-based vaccines targeted PRRSV-2, but not PRRSV-1 or both. Therefore, it is urgent to produce VLPs against PRRSV-1. In this study, we rescued recombinant baculovirus expressing GP5 and M proteins of PRRSV-1 through the Bac-to-Bac® baculovirus expression system. Thereafter, PRRSV VLP was obtained efficiently in the recombinant baculovirus-infected High Five insect cells. Moreover, the PRRSV VLP and PRRSV VLP+A5 could efficiently trigger specific humoral immune responses and B cellular immune responses through intranasal immunization. The combination of PRRSV VLP and A5 adjuvant could improve the level of the immune response. The PRRSV-1 VLPs generated in this study have greater potential for vaccine development to control PRRSV-1 infection.