Measurement of intact quantal packet of transmitters released from single nerve terminal by loose-patch amperometry
Measurement of intact quantal packet of transmitters released from single nerve terminal by loose-patch amperometry
复制标题
松散贴片电流法测量单神经末梢释放的发射机完整量子包
DOI：
10.1016/j.bios.2021.113143
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发表时间：
2021-06-01
影响因子：
12.6
通讯作者：
Sun, Jianyuan
中科院分区：
文献类型：
--
作者：
Chen, Peihua;Shen, Xuefeng;Sun, Jianyuan
神经元信息主要由突触化学编码，突触传递的基本单位是单个囊泡释放的神经递质。然而，目前对突触中量子神经递质的含量还没有精确的估计，这在很大程度上阻碍了我们对量子神经递质释放的本质及其对神经元信息处理的影响的理解。为了突破精确计数神经递质量子分子的技术瓶颈，我们发展了一种电生理学和电化学相结合的新方法来测量单个囊泡的完整量子含量。设计了一种用于电化学检测的蚀刻亚微米碳纤维电极，该电极封装在电生理用玻璃移液管中。玻璃移液管允许电化学电极进入释放部位，并在电生理学松散贴片模式下在封闭空间内进行安培计记录。我们的研究表明，完整的量子释放，可以成功地检测到多巴胺能静脉曲张，这种松散的补丁安培测量在真实的时间与可忽略不计的泄漏。
Neuronal information is majorly encoded chemically at synapses and the elementary unit of synaptic transmission is the contents of neurotransmitter released from single vesicle. However, the contents of quantal neurotransmitter have never been precisely estimated at synapses, which largely prevent our understanding the nature of quantal neurotransmitter release and its impact on neuronal information processing. In order to break through the technical bottleneck of precisely counting quantal neurotransmitter molecules, we developed a new approach in combination of electrophysiology and electrochemistry to measure intact quantal content of single vesicles. An etched submicro-carbon fiber electrode for electrochemical detection was designed to be enclosed in an electrophysiologically used glass pipette. The glass pipette allowed the electrochemical electrode to access the release site, and ampemmetric recordings were made within the enclosed space at the electrophysiological loose-patch mode. Our study showed that the intact quantal release could be successfully detected at the dopaminergic varicosities by this loose-patch amperometric measurement in real time with negligible leakage.