THE MODULATION OF SERINE METABOLISM IN HEPATOMA 3924A DURING DIFFERENT PHASES OF CELLULAR PROLIFERATION IN CULTURE
THE MODULATION OF SERINE METABOLISM IN HEPATOMA 3924A DURING DIFFERENT PHASES OF CELLULAR PROLIFERATION IN CULTURE
复制标题
DOI：
10.1042/bj2450609
复制
发表时间：
1987-07-15
影响因子：
4.1
通讯作者：
WEBER, G
中科院分区：
文献类型：
--
作者：
SNELL, K;NATSUMEDA, Y;WEBER, G
3-磷酸甘油酸脱氢酶和丝氨酸羟甲基转移酶的活性显着增加，在过渡的肝癌细胞从静止的非增殖培养到增殖生长期。随着细胞达到汇合并进入平台生长期，活性下降。这种模式被[14 C]丝氨酸掺入核酸的变化所取代。这些实验支持了这样的假设：丝氨酸的生物合成与其在癌细胞中形成核苷酸前体的利用在代谢上偶联。
The activities of 3-phosphoglycerate dehydrogenase and serine hydroxymethyltransferase increased markedly during the transition of hepatoma cells from a resting non-proliferating culture into the proliferating growth phase. Activities declined as cells reached confluency and entered the plateau growth phase. This pattern was paralleled by changes in [14C]serine incorporation into nucleic acids. The experiments support the hypothesis that the biosynthesis of serine is metabolically coupled to its utilization for nucleotide precursor formation in cancer cells.