Bone marrow stromal cells enhance differentiation of cocultured neurosphere cells and promote regeneration of injured spinal
Bone marrow stromal cells enhance differentiation of cocultured neurosphere cells and promote regeneration of injured spinal
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DOI：
10.1002/jnr.10587
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发表时间：
2003-05-01
影响因子：
4.2
通讯作者：
Ide, C
中科院分区：
文献类型：
--
作者：
Wu, SF;Suzuki, Y;Ide, C
骨髓基质干细胞（MSCs）移植已被认为是促进神经再生的潜在途径。本研究探讨了骨髓间充质干细胞对体外培养的脊髓神经球细胞的影响，以及骨髓间充质干细胞移植对损伤脊髓再生的影响。通过细胞混合或单层饲养层培养，将来自成年大鼠的MSC与胎儿脊髓源性神经球细胞共培养。在混合细胞培养中，神经鞘细胞被刺激以发育广泛的突起。在单层饲养层培养中，来自神经球细胞的许多突起似乎被MSC吸引。在体内实验中，移植的MSC通过增强损伤的组织修复来促进受损脊髓的再生，留下的空腔明显小于对照组。虽然移植的MSC的数量逐渐减少，但一些治疗的动物显示出显着的功能恢复。这些结果表明，MSCs可能对神经球细胞的分化有深远的影响，并能通过移植促进脊髓的再生。(C)2003 Wiley-Liss，Inc.
Transplantation of bone marrow stromal cells (MSCs) has been regarded as a potential approach for promoting nerve regeneration. In the present study, we investigated the influence of MSCs on spinal cord neurosphere cells in vitro and on the regeneration of injured spinal cord in vivo by grafting. MSCs from adult rats were cocultured with fetal spinal cord-derived neurosphere cells by either cell mixing or making monolayered-feeder cultures. In the mixed cell cultures, neuroshpere cells were stimulated to develop extensive processes. In the monolayered-feeder cultures, numerous processes from neurosphere cells appeared to be attracted to MSCs. In an in vivo experiment, grafted MSCs promoted the regeneration of injured spinal cord by enhancing tissue repair of the lesion, leaving apparently smaller cavities than in controls. Although the number of grafted MSCs gradually decreased, some treated animals showed remarkable functional recovery. These results suggest that MSCs might have profound effects on the differentiation of neurosphere cells and be able to promote regeneration of the spinal cord by means of grafting. (C) 2003 Wiley-Liss, Inc.